土壤微生物群落对连作种植制度的响应

利用高通量测序技术研究玉米和大豆连作对土壤微生物群落的影响。结果表明，与休闲地块相比，连作降低了真菌丰度和真菌物种多样性。大豆连作显著增加了子囊菌(Ascomycota)、孢霉菌(Mortierellomycota)的相对丰度，降低了担子菌(Basidiomycota)、球囊菌(Glomeromycota)的相对丰度。玉米连作增加了担子菌门相对丰度，降低了孢霉菌门(Mortierellomycota)、球囊菌的相对丰度。与休闲地土壤相比，玉米连作和大豆连作种植土壤中变形菌(Proteobacteria)、拟杆菌(Bacteroidetes)、绿弯菌(Chloroflexi)的相对丰度显著增加，放线菌(Actinobacteria)、芽单胞菌(Gemmatimonadetes)和浮霉菌(Planctomycetes)的相对丰度显著下降。大豆连作土壤中镰孢菌的相对丰度显著高，镰孢菌与子囊菌门显著相关。

Response of Soil Microbial Community to Continuous Cropping System

Continuous cropping causes continuous cropping obstacles, but the impact of different continuous cropping on soil microbial community is not clear. In this study, high-throughput sequencing technology was used to study the effects of continuous cropping of corn and soybean on soil microbial community. Compared with fallow plots, continuous cropping reduced fungal abundance and fungal species diversity. Soybean continuous cropping significantly increased the relative abundance of Ascomycota and Mortierellomycota, and decreased the relative abundance of Basidiomycota and Glomeromycota. The continuous cropping of maize increased the relative abundance of Basidiomycetes, but decreased the relative abundance of Mortierellomycota and Glomeromycota. Compared with the fallow soil, the relative abundance of Proteobacteria, Bacteroides and Chloroflexi increased significantly, while the relative abundance of Actinobacteria, Gemmatimonadetes and Planctomycetes decreased significantly. The relative abundance of Fusarium in soybean continuous cropping soil was significantly high, and Fusarium was significantly correlated with Ascomycetes.