EBR对盐胁迫下玉米幼苗生理指标及抗氧化酶和水孔蛋白基因表达量的影响

以玉米自交系Mo17为材料,用不同浓度的EBR处理150 mmol/L NaCl胁迫的玉米幼苗,测定其叶绿素含量、过氧化物酶(POD)活性、脯氨酸含量、可溶性糖含量、丙二醛(MDA)含量和根系活力等指标,同时通过荧光定量PCR测定CAT酶基因和ZMPIP2-4基因的表达.结果 表明,EBR处理后,玉米幼苗的POD酶活性...

Effects of Different Concentrations of EBR on Physiological Characteristics and the Expression Levels of CAT Enzyme and ZMPIP24 Genes in Maize Seedlings under Salt Stress

Maize inbred line Mo17 was used as the material, and its seedlings under 150mmol/L NaCl stress were treated with different concentrations of EBR. Their chlorophyll content, Peroxidase(POD) activity, proline content, soluble sugar content, and malondialdehyde(MDA) content and root vigor were measured, while the expression of CAT enzyme gene and ZMPIP2-4 gene was measured by fluorescence quantitative PCR. The results of the study showed that:After EBR treatment, the POD enzyme activity, soluble sugar content, proline content and root activity of corn seedlings increased; MDA content decreased. When the concentration of EBR treatment was 0.1 mg/L, soluble sugar, peroxidase, and root activity increased significantly. In addition, the expression of catalase(CAT) gene increased significantly at 0.001 mg/L. In summary, when the EBR concentration is 0.1mg/L, it can effectively increase the salt stress resistance of maize at the physiological level; when the EBR concentration is 0.001 mg/L, it can significantly increase the expression of CAT and ZMPIP2-4 genes in the salt stress resistance of maize seedlings at the genetic level. It can be concluded that the optimal EBR concentration to effectively alleviate salt stress is 0.1 mg/L. Physiological characteristics are the phenotypes of genes, but the specific mechanism of regulation after the expression of stress-resistant genes in maize seedlings is still unclear, so the further investigation is needed.