| 花魔芋组织培养初步研究 |
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| 引用本文： | 鲁红学,胡桂香,周燚,潘娜,黄小贞.花魔芋组织培养初步研究[J].长江大学学报,2005,2(5):52-54. |
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| 作者姓名： | 鲁红学 胡桂香 周燚 潘娜 黄小贞 |
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| 作者单位： | 长江大学农学院,湖北,荆州,434025;长江大学农学院,湖北,荆州,434025;长江大学农学院,湖北,荆州,434025;长江大学农学院,湖北,荆州,434025;长江大学农学院,湖北,荆州,434025 |
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| 摘 要： | 以花魔芋球茎切块作外植体,接种在MS附加6-BA、NAA和KT的培养基上诱导愈伤组织的形成.各种培养基均能诱导外植体产生愈伤组织,但不同激素浓度组合其诱导率不一样.其中以MS 6-BA 0.5 mg/L NAA 1.0 mg/L的诱导率最高,达84.62%.愈伤组织芽分化最适培养基是MS 6-BA 1.0 mg/L NAA 0.5 mg/L KT2.0 mg/L,分化率为73.81%.芽在MS NAA 0.5 mg/L和1/2MS NAA 0.5 mg/L培养基上的生根率均在78%以上.
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| 关 键 词： | 花魔芋 愈伤组织 分化培养 |
| 文章编号： | 1673-1409(2005)05-0052-03 |
| 修稿时间： | 2004年11月29日 |
Studies on Tissue Culture of Elephant-foot Yam (Amorphophallus rivieri Durieu) |
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| LU Hong-xue,HU Gui-xiang,ZHOU Yi,PAN Na,HUANG Xiao-zhen.Studies on Tissue Culture of Elephant-foot Yam (Amorphophallus rivieri Durieu)[J].Journal of Yangtze University,2005,2(5):52-54. |
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| Authors: | LU Hong-xue HU Gui-xiang ZHOU Yi PAN Na HUANG Xiao-zhen |
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| Abstract: | The corms of Amorphophallus rivieri Durieu,a species of the genus Amorphophallus,were used as explants for tissue culture.The corms could be all induced to form callus when cultured on MS_1 medium supplemented with different hormone concentrations,but the callus induction frequency was different,and the maximal frequency was 84.62% when cultured on MS+6-BA0.5 mg/L+NAA 1.0 mg/L.The callus was differentiated to bud on MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+KT 2.0 mg/L with differential rate being 73.81%.The rootage rate was both above 78% on the medium MS+ NAA (0.5 mg/L) and 1/2MS+ NAA 0.5 mg/L. |
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| Keywords: | elephant-foot yam(Amorphophallus rivieri Durieu) callus differentiation culture |
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