| 利用冷冻的GV期卵母细胞制备延边黄牛核移植胚胎的研究 |
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| 引用本文： | 王士勇,杨月春,方南洙,李钟淑,李福俊.利用冷冻的GV期卵母细胞制备延边黄牛核移植胚胎的研究[J].江西农业大学学报,2010,32(2). |
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| 作者姓名： | 王士勇 杨月春 方南洙 李钟淑 李福俊 |
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| 作者单位： | 延边大学,农学院,吉林,龙井,133400 |
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| 基金项目： | 国家自然科学基金(30860184);;延边大学“211工程”三期建设项目 |
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| 摘 要： | 研究使用玻璃化冷冻、程序化冷冻和超快速冷冻3种方法冷冻延边黄牛的卵母细胞,确定适宜的延边黄牛核移植受体卵母细胞的冷冻方法。结果显示,3种冷冻方法处理的GV期卵母细胞在成熟率上相似,玻璃化冷冻法得到的卵母细胞形态正常率要高于程序化冷冻和超快速冷冻法(P<0.05),超快速冷冻法回收率最低。采用玻璃化冷冻法,核移植重组胚卵裂率明显高于程序化冷冻和超快速冷冻法(P<0.05),在融合率上,3种方法差异不显著。表明玻璃化冷冻方法对延边黄牛GV期卵母细胞冷冻效果优于程序化冷冻和超快速冷冻方法,是一种较适合延边黄牛核移植的卵母细胞冷冻方法。
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| 关 键 词： | 延边黄牛 卵母细胞 冷冻保存 核移植 |
A Study on Preparation of Reconstructed Embryo in Yanbian Cattle Using Freezing GV Oocytes |
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| WANG Shi-yong,YANG Yue-chun,FANG Nan-zhu,LI Zhong-shu,LI Fu-jun.A Study on Preparation of Reconstructed Embryo in Yanbian Cattle Using Freezing GV Oocytes[J].Acta Agriculturae Universitis Jiangxiensis,2010,32(2). |
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| Authors: | WANG Shi-yong YANG Yue-chun FANG Nan-zhu LI Zhong-shu LI Fu-jun |
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| Institution: | Agricultural Colloge of Yanbian University/a>;Longjing 133400/a>;China |
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| Abstract: | In order to determine appropriate freezing method for oocyte used in nuclear transfer of Yanbian cattle,three methods were used to freeze oocyte,namely,vitrification,programmed and ultra-rapid freezing.The results showed that maturation rate of oocyte was similar in three treatment groups,morphologically normal rate of oocytes obtained from vitrification freezing method was higher than that from programmed freezing method and ultra-rapid freezing method (P<0.05),and the recovery rate was lowest in the ultra... |
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| Keywords: | Yanbian cattle oocyte cryopreservation nuclear transfer |
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