| 人Neurturin基因的克隆及序列分析 |
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| 引用本文： | 崔羽,李璐,李景鹏.人Neurturin基因的克隆及序列分析[J].东北农业大学学报,2008,39(1):71-74. |
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| 作者姓名： | 崔羽 李璐 李景鹏 |
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| 作者单位： | 海南大学生命科学学院,海口,570105;东北农业大学生命科学学院,哈尔滨,150030 |
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| 摘 要： | 文章以人胎脑cDNA为模板,采用巢式PCR方法扩增人Neurturin成熟蛋白基因,并将其插入到质粒pGEM-T,构建克隆载体pGEM-T-hNTN。对阳性重组子进行鉴定和序列测定,并与已克隆的人Artemin成熟蛋白基因进行同源性分析。结果表明,所克隆DNA片断与文献发表序列(GenBank NM004558)完全一致,人Neurturin成熟蛋白基因与Artemin同源性为58.5%。
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| 关 键 词： | Neurturin 基因克隆 序列分析 |
| 文章编号： | 1005-9369（2008）01-0071-04 |
| 收稿时间： | 2006-04-13 |
| 修稿时间： | 2006年4月13日 |
Cloning and sequence analysis of human Neurturin |
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| CUI Yu,LI Lu,LI Jingpeng.Cloning and sequence analysis of human Neurturin[J].Journal of Northeast Agricultural University,2008,39(1):71-74. |
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| Authors: | CUI Yu LI Lu LI Jingpeng |
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| Abstract: | With cDNA of human fetal brain as the template, the matured human Neurturin gene was amplified by nested PCR and inserted into the plasmid pGEM-T to construct the cloning vector pGEM-T-hNTN. The positive recombinant was identified and sequenced, and its homology of gene and protein with the matured human Artemin was analyzed. The results showed the sequence of the cloned DNA fragment was the same as the reported one (GenBank NM004558,(c) completely, and the homology between Neurturin and Artemin was 58.5%. |
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| Keywords: | Neurturin |
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