| 烟草cDNA-AFLP体系中选择性引物筛选 |
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| 引用本文： | 刘磊,郭兆奎,万秀清,颜培强,乔婵,刘丹.烟草cDNA-AFLP体系中选择性引物筛选[J].黑龙江八一农垦大学学报,2010,22(5):9-12. |
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| 作者姓名： | 刘磊 郭兆奎 万秀清 颜培强 乔婵 刘丹 |
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| 作者单位： | [1]牡丹江烟草科学研究所,牡丹江157011 [2]黑龙江八一农垦大学农学院,牡丹江157011 |
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| 基金项目： | 黑龙江省烟草专卖局资助项目 |
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| 摘 要： | 为确定cDNA-AFLP技术在烟草中的最适合的选择性碱基数目,本实验对低钾胁迫下的烟草根系进行了cDNA-AFLP分析,通过240对引物组合的筛选,共得到2100个转录衍生片段,并根据不同选择性碱基的组合对2100个片段进行了分析。结果表明,在扩增产物过多时,应将引物中选择性碱基的数量调整到3个较为合适。
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| 关 键 词： | 烟草 cDNA-AFLP 低钾胁迫 |
Selective Primer Screening in Nicotiana Tabacum cDNA-AFLP System |
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| Liu Lei,Guo Zhaokui,Wan Xiuqing,Yan Peiqiang,Qiao Chan,Liu Dan.Selective Primer Screening in Nicotiana Tabacum cDNA-AFLP System[J].Journal of Heilongjiang August First Land Reclamation University,2010,22(5):9-12. |
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| Authors: | Liu Lei Guo Zhaokui Wan Xiuqing Yan Peiqiang Qiao Chan Liu Dan |
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| Institution: | 1. Mudanjiang Tobacco Research Institute,Mudanjiang 157011;2. College of Agriculture,Heilongjiang Bayi Agricultural University) |
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| Abstract: | In ordre to determine the optimal selective bases number of cDNA-AFLP techniques in tobacco,cDNA-AFLP techniques was employed to identify genes differentially expressed in tobacco roots at low potassium. Through the selective amplification of 240 primer combinations,2 100 TDFs were obtained and they were analyzed according to different selective base combinations. The results showed that the number of the selective base in primer should be adjusted to 3 under excessive amplification. |
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| Keywords: | Nicotiana tabacum cDNA-AFLP Low Potassium Stress |
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