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Double haploids,markers and QTL analysis in vegetable brassicas
Authors:David Pink  Liz Bailey  Sandy McClement  Paul Hand  Evy Mathas  Vicky Buchanan-Wollaston  Dave Astley  Graham King  Graham Teakle
Institution:(1) Warwick HRI, Warwick University, Wellesbourne, Warwick, CV35 9EF, UK;(2) Rothamsted Research, West Common Harpenden, Hertfordshire, AL5 2JQ, UK
Abstract:Double haploid (DH) plants of Brassica spp. can be produced via anther culture or culture of microspores. This paper reviews the uses of double haploids in crop improvement research in vegetable brassicas (B. oleracea). Applications of DH lines are described for breeding; construction of linkage maps; genetic analysis of quantitative traits and capturing genetic variation. The advantages and disadvantages of DH lines are discussed
Keywords:Double haploids  Microspore culture  Anther culture  Brassicas  Linkage maps  Plant breeding
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