| 江口萝卜猪和梅山猪的猪应急综合征主效基因RYR1的PCR-RFLP检测 |
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| 引用本文： | 朱锋钊,杜秀园,冉雪琴.江口萝卜猪和梅山猪的猪应急综合征主效基因RYR1的PCR-RFLP检测[J].贵州畜牧兽医,2011,35(2):1-4. |
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| 作者姓名： | 朱锋钊 杜秀园 冉雪琴 |
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| 作者单位： | 1. 贵州省铜仁职业技术学院,贵州,铜仁,554300
2. 贵州大学动物科学学院,贵州,贵阳,550025 |
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| 基金项目： | 贵州省"十一五"农业重大专项科技攻关计划项目 |
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| 摘 要： | 猪兰尼定受体1(Ryanodine Receptor1,RYR1)基因是导致猪应激综合征、影响猪肉质的主效基因.试验采用RFLP方法,以大白猪、2个杂交猪群(杜洛克×长白猪×大白猪和长白猪×大白猪)为对照,检测了江口萝卜猪与引入的梅山猪群RYR1基因的分布类型.结果表明,对照组中,外三元、外二元杂交猪和大白猪中均检测到...
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| 关 键 词： | 江口萝卜猪、梅山猪 RYR1 PRC-RFLP 猪应激综合症 |
PCR-RFLP Analysis of Porcine Stress Syndrome RYR1 Gene for Jiangkou Radish Pig and Meishan Pig |
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| Zhu Fengzhao,Du Xiuyuan,Ran Xueqin.PCR-RFLP Analysis of Porcine Stress Syndrome RYR1 Gene for Jiangkou Radish Pig and Meishan Pig[J].Guizhou Animal Science and Veterinary Medicine,2011,35(2):1-4. |
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| Authors: | Zhu Fengzhao Du Xiuyuan Ran Xueqin |
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| Institution: | 1.Tongren Polytechnic,Guizhou Tongren 554300,China;2.College of Animal Science,Guizhou University,Guizhou Guiyang 550025,China) |
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| Abstract: | The Ryanodine Receptor1 gene in pig is the major gene lead to Porcine Stress Syndrome and has an effect on meat quality.The genotype distribution of Ryanodine Receptor1 gene was investigated in Luobopigand Meishan pig and taking Large White,and two hybrids(Duroc × Landrace × Large White,Landrace × Large White) as control by restriction fragment length polymorphism(RFLP) and sequencing analysis..The results showed that heterozygous(RYR1Nn) of RYR1 gene is detected in Meishan pig,and the allele n frequency of 5.56%,it shows that meishan pig invaded by the allele n frequency,it shoud be protect the meishan pig.And it no found the allele n gene,it shows that Luobo pig has favorable anti-stress ability.On the contrary,RYR1Nn genotype was determined at a higher rate of 5.77% in Large White and 11.11%,and 12.12% in two hybrids respectively. |
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| Keywords: | Luobo pig Meishan pig Ryanodine Receptor1 PRC-RFLP Porcine Stress Syndrome |
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