| 四倍体野生棉毛棉体细胞胚胎发生与植株再生研究 |
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| 引用本文： | 张巧,汪静儿,叶春丽,燕树峰,陈进红,洪彩霞,祝水金.四倍体野生棉毛棉体细胞胚胎发生与植株再生研究[J].棉花学报,2010,22(3):217-223. |
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| 作者姓名： | 张巧 汪静儿 叶春丽 燕树峰 陈进红 洪彩霞 祝水金 |
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| 作者单位： | 浙江大学农业与生物技术学院,杭州,310029 |
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| 基金项目： | 国家973计划项目(2004CB117305);;公益性行业(农业)科研专项经费项目(3-19);;国家自然科学基金(30671325、30700511) |
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| 摘 要： | 通过对培养基激素配比,以及不同浓度的PEG、CuSO4和AgNO3等处理,对棉属四倍体野生棉种毛棉(G.tomentosum Nutt.Watt)进行了体细胞培养,获得了大量体细胞胚状体并实现植株再生。与四倍体陆地棉(G.hirsutum,cv.Coker 201)相比,毛棉体细胞培养难度较大,且体细胞胚畸形严重,萌发困难,但通过培养条件的调控可以得到大量胚状体和少量再生植株。试验结果表明,激素组合0.1 mg.L-1 KT+0.1 mg.L-1 2,4-D诱导的愈伤组织较松脆,分化潜力高;PEG处理不利于毛棉的胚性和非胚性愈伤的诱导,而不同浓度的CuSO4和AgNO3处理对于体细胞胚状体诱导和萌发以及植株再生效果有明显差异。其中3.5 mg.L-1 CuSO4+0.5 mg.L-1IBA+0.2 mg.L-1 KT和3 mg.L-1 AgNO3+0.5 mg.L-1 IBA+0.2 mg.L-1 KT组合利于胚性愈伤的增殖和体细胞胚的萌发。
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| 关 键 词： | 毛棉 体细胞胚胎发生 硫酸铜 硝酸银 PEG |
| 收稿时间： | 2009-11-10; |
Somatic Embryogenesis and Plant Regeneration in Wild Cotton Species of G. Tomentosum |
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| ZHANG Qiao,WANG Jing-er,YE Chun-li,YAN Shu-feng,CHEN Jin-hong,HONG Cai-xia,ZHU Shui-jin.Somatic Embryogenesis and Plant Regeneration in Wild Cotton Species of G. Tomentosum[J].Cotton Science,2010,22(3):217-223. |
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| Authors: | ZHANG Qiao WANG Jing-er YE Chun-li YAN Shu-feng CHEN Jin-hong HONG Cai-xia ZHU Shui-jin |
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| Institution: | Agronomy Department/a>;College of Agriculture and Biotechnology/a>;Zhejiang University/a>;Hangzhou 310029/a>;China |
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| Abstract: | The wild cotton species of G.tomentosum has some outstanding characters such as the higher resistance to pests and tolerance to drought.However,the upland cotton germplasm with the trait has not been obtained through sexual hybridization between G.hirsutum and G.tomentosum due to the distant relationship between the wild species and upland cotton.Protoplast fu-sion technique which depends on the success in the somatic culture of two different species can avoid sexual incompatibility.The callus inducing and ... |
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| Keywords: | G tomentosum somatic embryogenesis CuSO4 AgNO3 PEG |
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