牛粪堆肥中添加抑菌剂对大肠杆菌杀灭效果的研究

目的]研究牛粪堆肥中添加抑菌剂（石灰氮）对大肠杆菌的杀灭效果,为牛粪的无害化处理提供理论依据。方法]试验组添加2.0%的抑菌剂,对照组不加抑菌剂,分别在不同的温度（20、303、75、06、0℃）条件下进行堆肥试验,确定适宜堆肥温度;并在30℃条件下添加不同剂量（0、2.0%、2.5%、3.0%）的抑菌剂,确定抑菌剂的最适添加量。结果]305、0、60℃是杀灭大肠杆菌较理想的温度;在30℃条件下,添加2.5%和3.0%的抑菌剂可在堆肥48 h时检测不到大肠杆菌,说明为达到杀灭牛粪中大肠杆菌的目的至少要在牛粪堆肥中添加2.5%的抑菌剂。结论]在堆肥（特别是冬季堆肥）中添加石灰氮对提高牛粪中大肠杆菌杀灭效果具有重要意义。

Sterilization Effects of Bacterial Inhibitor on Escherichia coli in Cattle Manure Compost

Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor(CaCN2) during the process of cattle manure composting so as to provide a theoretical basis for cattle manure harmless treatment.Method] Both experimental groups supplemented with 2.0% bacterial inhibitor and control groups without bacterial inhibitor were cultured at different temperatures(20,30,37,50,60 ℃) to determine the optimal composing temperature.Under 30 ℃,different doses(0,2.0%,2.5%,3.0%) of bacterial inhibitor were added into the compost to obtain the optimal bacterial inhibitor addition dose.Result] 30,50 and 60 ℃ were ideal temperatures for sterilization of E.coli.Under 30 ℃,E.coli couldn't be detected in 2.5% dose group and 3.0% dose group after culture for 48 h,demonstrating no less than 2.5% bacterial inhibitor should be added.Conclusion] It has an important significance to enhance the sterilization effects on E.coli by adding CaCN2 into cattle manure compost especially in winter.