大豆根瘤菌的分离与分子鉴定

为进一步发掘新的根瘤菌资源,丰富和充实根瘤菌资源库,本研究采用平板分离的方法从大豆根瘤中分离纯化根瘤菌,获得的菌株进行盆栽回接试验,结果表明分离获得的5株根瘤菌代表菌株均可在大豆上结瘤,具有结瘤能力。根据已公布大豆根瘤菌共同结瘤基因nodA保守区域设计引物对其进行了分子鉴定均获得了nodA PCR扩增产物,从而在分子水平上实现了对根瘤菌的快速鉴定。

Isolation and Molecular Identification of Soybean Rhizobia

In order to discover new soybean rhizobia resource and screen highly efficient soybean rhizobia for our region, soybean rhizobia was isolated and purified from soybean nodule by plating and the strains isolated were used to carry out pot culture experiment. Results showed that all the five representative strains gained could nodulate in soybean plant. Therefore all of them had the ability of nodulation. PCR amplification of common nodulation gene nodA was carried out using primer designed acoording to the conserved region of nodA. All the representative strains got nodA PCR products. Therefore the characteristics of strain nodulation belonging to rhizobia could be judged quickly from nodA at the level of molecule.