Identification and characterization of the <Emphasis Type="Italic">Enterobacter</Emphasis> complex causing mulberry (<Emphasis Type="Italic">Morus alba</Emphasis>) wilt disease in China |
| |
Authors: | Guo-Fen Wang Guan-Lin Xie Bo Zhu Jun-Sheng Huang Bo Liu Praphat Kawicha Lesley Benyon Yong-Ping Duan |
| |
Institution: | (1) State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, P. O. Box 3-398, Hangzhou 310029 Zhejiang province, People’s Republic of China;(2) Institute of Environmental and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737 Hainan province, People’s Republic of China;(3) Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003 Fujian province, People’s Republic of China;(4) Faculty of Natural Resources and Agro-Industry Kasetsart University, Sakonnakhon Province, 47000, Thailand;(5) USDA-ARS-USHRL, Fort Pierce, FL 34945, USA; |
| |
Abstract: | Mulberry wilt disease (MWD) was recently identified in Hangzhou, Zhejiang province, China. Typical symptoms of the disease
are browning of vascular tissues, leaf wilt, defoliation, and tree decline. Unlike the symptoms of bacterial wilt disease
caused by Ralstonia solanacearum, symptoms of MWD generally started from the bottom of the plants and moved upward. In inoculation experiments, four selected
MWD strains caused mulberry shoot leaf wilt, discoloration, and defoliation. They also induced whole plant leaf wilt, defoliation
and dark brown discoloration of vascular tissue. Based on Biolog metabolic profiles, fatty acid methyl ester analysis (FAME)
and sequence analysis of the partial 16S rDNA and rpoB genes four MWD strains were identified as members of the genus Enterobacter. The 16S rDNA and rpoB gene sequences revealed a close relationship among two isolates, R2-2 and R6-2, and the E. asburiae type strain JCM6051. The isolates showed >98% similarity to E. asburiae JCM6051 in their rpoB gene. These results indicated that isolates R2-2 and R6-2 belonged to E. asburiae. No similarity in 16S rDNA sequences above 97% was found between either of the remaining isolates, R11-2 or R18-2, and any
recognized Enterobacter species, suggesting that the two isolates may represent novel Enterobacter species. rpoB gene similarity values between the isolates and Enterobacter spp. type strains were <98%, providing further evidence that the two isolates may represent a novel species within the Enterobacter. The causal agent for MWD was previously reported to be E. cloacae, however, this study found that other Enterobacter spp. (E. asburiae and Enterobacter sp.) also cause MWD. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|