厚朴酚在体外对LPS诱导细胞因子释放及ERK通路激活的影响

利用MTT方法检测厚朴酚细胞毒性，ELISA方法检测厚朴酚对LPS介导炎症中IL-1、IL-6、IL-10释放的调节作用，Western—blot方法检测对ERK1／2活化的调节作用。结果显示，LPS处理过的RAW264．7细胞在经过不同剂量厚朴酚处理后，其IL-1、IL-6的释放随厚朴酚剂量的增加在减少，而IL-10则随药物剂量增加而增加；厚朴酚对LPS介导的ERK／MAPK通路激活作用显示一定的抑制作用。结果表明，在一定剂量范围内厚朴酚可以缓解LPS刺激的细胞因子的释放，在炎症过程中可以通过抑制ERK／MAPK活化来抑制炎症发生。

Magnolol inhibits LPS-induced cytokine releases and the activation of ERK pathway in vitro

The cytotoxicity of magnolol alone and in the presence of LPS to RAW264.7 cells,was tested by MTT assay.The releases of IL-1,IL-6 and IL-10 of LPS-stimulated RAW264.7 cells were measured by ELISA.The activation of ERK1/2 pathway was tasted by Western-blot.Magnolol reduced releases of IL-1 and IL-6,meanwhile increased release of IL-10 in RAW264.7cells stimulated by LPS.Via Weastern-blot assay,magnolol showed a significant inhibition on ERK/MAPK pathway activation.And the effects were in a dose-dependent manner.It indicated that magnolol perfored significant effects on LPS-induced inflammation in vitro by down-regulating pro-inflammatory factors and up-regulating anti-inflammatory factors.It also showed a significant effect on suppressing ERK1/2 pathway activation.