Antitumor activity of Dioscorea bulbifera L. rhizome in vivo |
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Authors: | Wang Jun-Ming Ji Li-Li Branford-White Christopher J Wang Zai-Yong Shen Kai-Kai Liu Hai Wang Zheng-Tao |
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Affiliation: | The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China. |
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Abstract: | Antitumor activities of water extract (fraction A), ethanol extract (fraction B), ethyl acetate extract (fraction C), non-ethyl acetate extract (fraction D) and compound diosbulbin B isolated from Dioscorea bulbifera L. (DB) were investigated in vivo in this present study. The results showed that fractions B and C both decreased tumor weight in S180 and H22 tumor cells bearing mice, while fractions A and D had no such effect. Furthermore, fraction C altered the weight of spleen and thymus, and the amounts of total leukocytes, lymphocytes and neutrophils in tumor-bearing mice. Further results showed that compound diosbulbin B demonstrated anti-tumor effects in the dose-dependent manner at the dosage of 2 to 16 mg/kg without significant toxicity in vivo. Furthermore, on the basis of chemical analysis of the above extracts by high-performance liquid chromatography (HPLC) with a diode array detector (DAD), diosbulbin B was found to be the major antitumor bioactive component of DB. These results suggest that DB has potential anti-tumor effects which may be related to influencing the immune system for the first time, and the compound diosbulbin B is the major antitumor component of DB. |
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Keywords: | DB, Dioscorea bulbifera L. fraction A, water extract fraction B, ethanol extract fraction C, ethyl acetate extract fraction D, non-ethyl acetate extract 5-Fu, 5-Fluorouracil i.g., intragastric administration CMC-Na, sodium carboxymethyl cellulose ALT, alanine transaminase AST, aspartate transaminase DAD, diode array detector HPLC, high-performance liquid chromatography S.E.M., standard error of mean SNK, Student Newman Keuls SPSS, Statistics Package for Social Science. |
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