撑篙竹遗传变异的RAPD分析

采用随机扩增多态DNA(RAPD)方法对6个群体30丛撑篙竹个体进行了遗传变异的研究。28个随机引物共检测到173个位点,其中85个是多态位点,平均每个引物提供6．18个RAPD信息量,扩增出的DNA片段大小一般在200～2000bp范围之间;用POPGENE1．31版软件进行遗传多样性分析：平均Nei’s基因多样性为0．2114,Shannon’s信息指数为0．3277,基因分化系数0．1853,表明群体间有一定的分化;各群体平均遗传距离0．0350,表明群体亲缘关系较近;试用UPGMA方法对不同产地的撑篙竹群体作聚类分析,初步可将6个群体聚为3类。

RAPD Analysis on Genetic Variation of Bambusa pervariabilis McClure

Thirty clumps of six populations of Bambusa pervariabilis were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variations among and within the populations. A total of 173 loci including 85 polymorphic loci were amplified using 28 random primers, with an average of 6.18 fragments each primer. The length of fragments was between 200 bp and 2 000 bp. As analyzed by POPGENE version 1.31, the data from six populations had an average Nei's gene diversity of 0.211 4, and Shannon's genetic diversity of 0.327 7, coefficient of gene differentiation(Gst) of 0.185 3, indicating that there was some differentiation among the populations. The average genetic distance among populations was 0.035 0, indicating that there was close relative relations among populations. The six populations were clustered to three categories by cluster analysis (UPGMA) based on Nei's unbiased genetic distance.