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Isolation and Characterization of Pepsin-Soluble Collagen from the Skin of Peru Squid (Dosidicus gigas) |
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| Authors: | Wandong Fu Yan Wang Bin Zheng Miaofei Liao Weijie Zhang |
| Affiliation: | 1. Zhejiang Marine Development Research Institute , Zhoushan , P. R. China wandongfu@126.com;3. Zhejiang Ocean University , Zhoushan , P. R. China;4. Zhejiang Marine Development Research Institute , Zhoushan , P. R. China;5. Lanzhou University of Technology , Lanzhou , P. R. China |
| Abstract: | Pepsin-soluble collagen (PSC) was isolated from Peru squid (Dosidicus gigas) skin and physicochemical properties of the PSC were determined. The PSC exhibited a maximum absorbance at 220 nm. Sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the collagen containing α1 and α2 chain was classified as type I collagen. Amino acid composition indicated that the collagen had lower amino acid content than that of mammalian collagen. Denaturation temperature (Td) of the PSC was 26.8°C. The PSC had relatively high solubility in alkaline condition or NaCl concentrations below 2%. Fourier transform infrared spectroscopy investigations showed the existence of helical arrangements of collagen. The lyophilized collagen had a uniform and regular network structure. These results suggested that Peru squid skin was a potential source of collagen for further research and application. |
| Keywords: | Peru squid pepsin-soluble collagen isolation characterization |