Optimization of Hydrolysis Conditions for Production of Angiotensin-Converting Enzyme Inhibitory Peptides from Basa Fish Skin Using Response Surface Methodology |
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Authors: | Yuhao Zhang Liang Ma Jeanette Otte |
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Institution: | 1. College of Food Science, Southwest University, Beibei, Chongqing, P.R. Chinazhy1203@163.com;3. College of Food Science, Southwest University, Beibei, Chongqing, P.R. China;4. Faculty of Science, University of Copenhagen, Copenhagen, Denmark |
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Abstract: | ABSTRACTBasa fish skin free from fat and noncollagenous proteins was hydrolyzed by different proteinases to produce angiotensin-converting enzyme (ACE) inhibitory peptides. Protamex hydrolysates were found to have the highest ACE inhibitory activity. Response surface methodology (RSM) was applied to optimize the hydrolysis conditions of Protamex—including enzyme-to-substrate ratio, hydrolysis time, and solid (fish skin)-to-liquid (water for hydrolysis)—at a temperature of 50°C and a pH of 7.0. The mathematical model demonstrated a good fit with the experimental data. An enzyme-to-substrate ratio of 6,108 U/g, a hydrolysis time of 1.24 h, and a solid-to-liquid ratio of 1:8.05 g/mL were found to be the optimal conditions to achieve the highest value of ACE inhibitory activity. The IC50 of the hydrolysates for ACE inhibitory activity was 1.417 mg/mL under these conditions. |
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Keywords: | Basa fish skin collagen hydrolysis peptide ACE inhibition response surface methodology |
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