The Feasibility of Increasing Lipid Extraction in Tilapia (Oreochromis niloticus) Waste by Proteolysis |
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Authors: | Ligianne Din Shirahigue Maira Oliveira Silva Aline Cristina Camargo Lia Ferraz de Arruda Sucasas Ricardo Borghesi Ingridy Simone Ribeiro Cabral |
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Affiliation: | 1. Center of Nuclear Energy in Agriculture, University of S?o Paulo, Piracicaba, S?o Paulo, Brazilligianneds@yahoo.com.br;3. Luiz de Queiroz College of Agriculture, University of S?o Paulo, Piracicaba, S?o Paulo, Brazil;4. Brazilian Agricultural Research Corporation, Corumbá, Mato Grosso do Sul, Brazil;5. Faculty of Pharmaceutical Sciences, Laboratory of Phytochemistry and Medicinal Chemistry, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil |
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Abstract: | ABSTRACTThe aim of the present study was to evaluate the feasibility of increasing lipid extraction in tilapia (Oreochromis niloticus) waste using different enzymes and to minimize the periods of hydrolysis to evaluate the performance of the lipid fractions. When processing waste tilapia, commercial enzymes were added that were composed of neutrase and alcalase acting at different hydrolysis times to produce the best yields. The treatment proved feasible for obtaining the protein hydrolysates. The type of enzyme and the hydrolysis time determined the degree of fractionation. The enzymes performed well, and the highest efficiency occurred within 2 h of hydrolysis. By fractionating, the obtained by-products can be applied in the preparation of feed, as the lipid fraction treatments yielded significant amounts of polyunsaturated fats and suitable n-6/n-3 ratios. |
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Keywords: | Waste tilapia yield lipid fraction hydrolysis |
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