| Optimization of Fermentation Condition of Yeast Culture |
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| 作者单位： | WANG Qiuju1，XU Li1*，and CUI Yizhe2 1 College of Animal Science and Technology，Northeast Agricultural University，Harbin 150030，China 2 College of Animal Science and Technology，Heilongjiang August 1st Agricultural University，Daqing 163319，Heilongjiang，China |
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| 基金项目： | Supported by Program of Harbin Tackle Key Problem （2004AA6BNO20） |
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| 摘 要： | Culture condition of every phase for fermentation of yeast culture was studied, and its solid and liquid conditions of elaboration were optimized to improve the total counts of living cells. Results showed that microzyme grew best at 30℃ when solid fermented, and the count of the living cells reached the tiptop with pH 5.5. The count of Candida tropicalis could reach 137.96× 10^9 cfu·g^-1, the count of Saccharomyces cerevisia could reach 134.62× 10^9 cfu·g^-1 the best liquid fermentation condition for cell-wall broken was 50℃ for 28 h, the rate of cell-wall broken could reach 80% at least; the rate of vitamin loss in yeast could be the minimun, the loss rate of vitamin B6 in Candida tropicalis and Saccharomyces cerevisiae was 8.71% and 19.54% respectively, the loss rate of vitamin B2 was 19.39% and 13.18%, respectively, and the loss rate of vitamin B6 was 6.3% and 3.04%, respectively.
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| 关 键 词： | 发酵条件 酵母 最优化分析 温度 |
Optimization of Fermentation Condition of Yeast Culture |
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| Authors: | WANG Qiuju XU Li CUI Yizhe |
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| Institution: | 1. College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China
2. College of Animal Science and Technology,Heilongjiang August Ist Agricultural University, Daqing 163319, Heilongjiang, China |
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| Abstract: | Culture condition of every phase for fermentation of yeast culture was studied, and its solid and liquid conditions of elaboration were optimized to improve the total counts of living cells.Results showed that microzyme grew best at 30℃ when solid fermented,and the count of the living cells reached the tiptop with pH 5.5.The count of Candida tropicalis could reach 137.96×109 cfu·g-1,the count of Saccharomyces cerevisia could reach 134.62×109 cfu·g-1;the best liquid fermentation condition for cell-wall broken was 50℃ for 28 h,the rate of cell-wall broken could reach 80% at least;the rate of vitamin loss in yeast could be the minimun, the loss rate of vitamin B1 in Candida tropicalis and Saccharomyces cerevisiae was 8.71% and 19.54% respectively, the loss rate of vitamin B2 was 19.39% and 13.18%,respectively,and the loss rate of vitamin B6 was 6.3% and 3.04%,respectively. |
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| Keywords: | yeast culture fermentation temperature acidity cell-wall broken |
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