Responses of antioxidant enzymes in the needles of hinoki cypress (Chamaecyparis obtusa) seedlings to nutrient solutions containing various calcium/aluminum ratios |
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Authors: | Tadashi Ogawa Chisato Takenaka Takafumi Tezuka |
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Institution: | (1) Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601 Nagoya, Japan;(2) Graduate School of Human Informatics, Nagoya University, 464-8601 Nagoya, Japan |
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Abstract: | An attempt was made to examine the possible connection between the various ratios of calcium/aluminum (Ca/Al) in the nutrient
solution of plant cultures and the active oxygen scavenging system of hinoki cypress (Chamaecyparis obtusa) seedlings. The hinoki cypress seedlings were transferred to nutrient solutions containing 5 mM AlCl3 together with various concentrations of Ca(NO3)2 in pots containing glass beads and Teflon tips. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase
(APX) and glutathione reductase (GR) in the needles were estimated at several stages during the 7-day treatment. The samples
treated with the lower Ca/Al solutions show the highest SOD activities. The activities of APX and GR, both of them involved
in the ascorbate-glutathione cycle, showed the same tendency with decrease to higher Ca/Al ratio. These results indicate that
rhizospheric Ca might compete with Al and ameliorate Al toxicity on and in the roots, the Al stress is not transformed to
the needles after a few days, and the ascorbate-glutathione cycle in the hinoki cypress needles might fluctuate and be suppressed
by the rhizospheric Al stress during the 7 days.
This work was supported in part by funding from the Japan Science & Technology Corporation, the CREST program 1996–2001, and
the Center for Forest Decline Studies. |
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Keywords: | ascorbate peroxidase (APX) catalase (CAT) glutathione reductase (GR) hinoki cypress (Chamaecyparis obtusa) superoxide dismutase (SOD) |
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