| RP-HPLC法测定甘草浸膏中甘草次酸含量 |
| |
| 引用本文： | 吴瑛,杨玲,卢士林.RP-HPLC法测定甘草浸膏中甘草次酸含量[J].塔里木农垦大学学报,2006,18(3):34-36. |
| |
| 作者姓名： | 吴瑛 杨玲 卢士林 |
| |
| 作者单位： | 1. 塔里木大学文理学院,新疆,阿拉尔,843300
2. 新农开发股份有限公司,新疆,阿拉尔,843300 |
| |
| 基金项目： | 塔里木大学校长基金资助项目(编号:200406) |
| |
| 摘 要： | 方法采用反相高效液相色谱法,以甲醇80%和水20%(用磷酸调PH为3.7)为流动相,以C8为分析柱,紫外检测波长为250nm,柱温室温,结果线性范围:0.01mg/m l~0.13mg/m l,线性方程A=50.535 31130.2C,相关系数0.99989;回收率101.85%,相对标准偏差1.47%。该方法目的在于测定甘草浸膏的甘草次酸含量,以期为检测评价甘草浸膏质量提供了指标,该方法方法简单、准确性高。
|
| 关 键 词： | 甘草浸膏 甘草次酸 反相高效液相色谱 |
| 文章编号： | 1009-0568(2006)03-0034-02 |
| 修稿时间： | 2005年12月15 |
The Quantitative Determination of Glycyrrhetinic Acid in Powdered Extracted Glycyrrhizin by RP-HPLC |
| |
| Wu Ying,Yang Lin,Lu Shiling.The Quantitative Determination of Glycyrrhetinic Acid in Powdered Extracted Glycyrrhizin by RP-HPLC[J].Journal of Tarim University of Agricultural Reclamation,2006,18(3):34-36. |
| |
| Authors: | Wu Ying Yang Lin Lu Shiling |
| |
| Abstract: | Aiming To develop a method to determine quantitatively the contents of glycyrrhetinic acid in Powdered Extractor Glycyrrhizae by RP-HPLC.RP-HPLC was used to quantitatively determine the contents of glycyrrhetinic acid.During experiments,the detection wavelength was 250nm room temperature.Results were good liner relations and the relative peak area of glycyrrhetinic acid r=0.99989.The average recovery of glycyrrhetinic acid was 101.85%,RSD1.47%.Conclusion: The method was simple,accurate and quick. |
| |
| Keywords: | powdered extracted glycyrrhizae Glycyrrhizic acid reversed phase high performance liquid chromatography(RP-HPLC) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
