Patterns of RFLP-based genetic diversity in germplasm pools of common wheat with different geographical or breeding program origins |
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Authors: | HS Kim RW Ward |
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Institution: | (1) Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI, 48824, U.S.A. |
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Abstract: | A set of 292 accessions of common wheat (Triticum aestivum L.) representing 21 germplasm pools based on geographical or breeding program origins was assayed for RFLP diversity. Thirty
cDNA and genomic DNA probes and the HindIII restriction enzyme were employed for RFLP analysis. About 61% of all 233 scored bands were present in 75% or more of
the accessions. All but one of the 30 probes revealed polymorphism, and the average number of distinct patterns per probe
over all accessions was 9.5.Polymorphic Information Content (PIC) values within a pool varied from 0 to 0.9 and depended on
the identities of both the germplasm pool and the probe. Rare banding patterns with a relative frequency of ≤0.2 within a
pool were detected. These rare patterns were more likely to occur in pools exhibiting high levels of heterogeneity. The highest
level of polymorphism was observed in the Turkish landraces from Southwest Asia. The Eastern U.S. soft red winter wheat germplasm
pool was more genetically diverse than the other advanced germplasm pools, and nearly as diverse as the Turkish landrace pool.
RFLP-based genetic relationships between germplasm pools generally tracked expectations based on common geographical origin,
breeding history and/or shared parentages. The Chinese wheat landraces from Sichuan, Tibet, and Yunnan provinces were distinct
from other pools. Similarity matrices for among-pool genetic distance estimates based on either band frequencies or banding
pattern frequencies showed good correlation with matrices derived from Nei and Li's mean genetic similarity estimates (r=−0.82** and r=−0.73**, respectively.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | genetic diversity germplasm RFLP Triticum aestivum wheat breeding |
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