| 杂交诱捕实时荧光PCR检测番茄环斑病毒 |
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| 引用本文： | 赵文军,陈红运,朱水芳,谭天伟. 杂交诱捕实时荧光PCR检测番茄环斑病毒[J]. 植物病理学报, 2007, 37(6): 666-669 |
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| 作者姓名： | 赵文军 陈红运 朱水芳 谭天伟 |
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| 作者单位： | 1 中国检验检疫科学研究院, 北京 100029;2 北京化工大学, 北京 100029 |
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| 基金项目： | 国家自然科学基金;科技部科研条件工作项目 |
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| 摘 要： | 番茄环斑病毒(Tomato ringspot nepovirus,ToRSV)广泛分布于欧美及大洋洲,能侵染葡萄、桃、苹果、樱桃、番茄及烟草等150多种植物,引起多种病害,严重时导致绝产[1]。
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| 关 键 词： | 实时荧光PCR检测 番茄环斑病毒 杂交 检疫性有害生物 PCR产物 特异性检测 诱捕 电泳技术 |
| 文章编号： | 0412-0914（2007）06-0666-04 |
| 收稿时间： | 2006-12-27 |
| 修稿时间： | 2007-04-09 |
Tomato ringspot nepovirus detection using hybridization capture real-time PCR |
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| ZHAO Wen-jun,CHEN Hong-yun,ZHU Shui-fang,TAN Tian-wei. Tomato ringspot nepovirus detection using hybridization capture real-time PCR[J]. Acta Phytopathologica Sinica, 2007, 37(6): 666-669 |
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| Authors: | ZHAO Wen-jun CHEN Hong-yun ZHU Shui-fang TAN Tian-wei |
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| Affiliation: | 1 Chinese Academy of Inspection and Quarantine, Beijing 100029, China;2 Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | A new virus detection method was developed by combining hybridization capture and real-time PCR technology with the sample of Tomato ringspot nepovirus.High purified DNA or RNA was crucial for the real-time PCR detection as the inhibitors of PCR reaction in template and might cause the false-negative cases.A probe was covalent bound to the surface of PCR tube for the DNA capture followed by real-time PCR detection.The steps of nucleic acids preparation and detection were both done in one the same tube.This method reduced the possibility of contamination as well increased the detection efficiency.It can be applied in plant virus detection especially for the sample with many inhibitors of PCR reaction. |
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| Keywords: | hybridization capture real-time PCR Tomato ringspot nepovirus detection |
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