| GFP与GnRH/TRS融合基因表达载体的构建及表达 |
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| 引用本文： | 金元昌,李璐,李景鹏. GFP与GnRH/TRS融合基因表达载体的构建及表达[J]. 动物医学进展, 2005, 26(11): 84-87 |
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| 作者姓名： | 金元昌 李璐 李景鹏 |
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| 作者单位： | 1. 湖南科技大学生命科学学院,湖南湘潭,411201
2. 东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
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| 摘 要： | 应用基因工程技术构建GnRH/TRS与绿色荧光蛋白的融合基因,核酸序列测定和Western boltting印迹分析基因表达;激光共聚焦荧光显微镜观察活细胞内荧光布局。结果融合基因获得了正确表达,GnRH/TRS-GFP融合基因的瞬间和稳定表达均获得了相同结果。GnRH/TRS-GFP融合蛋白具有绿色荧光蛋白的自发荧光特性,且不影响Gn-RH/TRS分子在细胞内的正确表达,为低促性腺激素性功能减退综合征治疗的进一步研究奠定了基础。
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| 关 键 词： | 绿色荧光蛋白 融合分子 转运肽 |
| 文章编号： | 1007-5038（2005）11-0084-04 |
| 收稿时间： | 2005-09-01 |
| 修稿时间： | 2005-09-01 |
Construction of Expression Vector and Expression of GFP and GnRH/TRS Gene Sequence |
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| JIN Yuan-chang,LI Lu,LI Jing-peng. Construction of Expression Vector and Expression of GFP and GnRH/TRS Gene Sequence[J]. Progress In Veterinary Medicine, 2005, 26(11): 84-87 |
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| Authors: | JIN Yuan-chang LI Lu LI Jing-peng |
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| Affiliation: | 1. Hunan University of Science and Technology , Xiangtan, Hunan, 411201, China; 2. Northeast Agricultural University , Harbin, Heilongjiang , 150030, China |
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| Abstract: | A fusion gene composed of green fluorescent protein(GFP) and GnRH/TRS DNA was constructed by standard cloning technology.Nucleotid sequence and Western blot analysis were used to evaluate the expression of the fused gene.Laser-scanning confocal microscopy was used to monitor the distribution of green fluorescence.Laser-scanning confocal microscopy clearly revealed that GnRH/TRS-GFP could be transiently and stably expressed in mammalian cells.The GFP tag did not interfere with the natural assembly and transport of GnRH/TRS molecule,and GnRH/TRS-GFP could act as a potential tool to learn more about GnRH/TRS molecules in living cells for further foundations established in the scientific research and the real application of treat of Gonadotropin-releasing hormone difficiency in the human(idiopathic hypogonadotropic hypogonadism and kallman's syndrom). |
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| Keywords: | GnRH |
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