| 牛源羊创伤球菌的分离鉴定 |
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| 引用本文： | 周伟,李能章,魏学良,胡娟,夏天,王自力,彭远义.牛源羊创伤球菌的分离鉴定[J].中国预防兽医学报,2012,34(7):584-586. |
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| 作者姓名： | 周伟 李能章 魏学良 胡娟 夏天 王自力 彭远义 |
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| 作者单位： | 西南大学动物科技学院重庆市牧草与草食家畜重点实验室,重庆,400715 |
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| 基金项目： | 国家现代农业(肉牛牦牛)产业技术体系建设专项基金 |
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| 摘 要： | 本实验从重庆某肉牛场肺炎患牛肺脏中分离一株革兰氏阳性双球菌,为进一步确定该菌株的分类地位,本研究对其进行细菌学分类鉴定。通过细菌培养特性、菌落形态观察、生化试验和PCR鉴定等研究表明,该分离菌与羊创伤球菌(H.ovis)(CCUG37441)特性相近;其16S rRNA序列与H.ovis标准株同源性达99.8%。药物敏感性试验和动物试验表明,该菌对菌必治最敏感,不致死小白鼠。
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| 关 键 词： | 牛 羊创伤球菌 分离鉴定 16S rRNA |
Isolation and identification of Helcococcus ovis from bovine |
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| ZHOU Wei
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LI Neng-zhang
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WEI Xue-liang
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HU Juan
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XIA Tian
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WANG Zi-li
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PENG Yuan-yi.Isolation and identification of Helcococcus ovis from bovine[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(7):584-586. |
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| Authors: | ZHOU Wei LI Neng-zhang WEI Xue-liang HU Juan XIA Tian WANG Zi-li PENG Yuan-yi |
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| Institution: | (Chongqing Key Laboratory of Forage & Herbivore,College of Animal Science & Technology,Southwest University,Chongqing 400715,China) |
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| Abstract: | A gram-positive diplococcus was isolated from a lung tissue sample with pneumonia in a beef cattle farm of Chongqing.The isolates was identified as Helcococcus ovis by the cultural,bacteria morphological observation,biochemical characteristics and the 16S rRNA sequence analysis which was 99.8% homology with standard strain of H.ovis.The drug susceptibility and pathogenicity tests showed that the isolate was sensitive to Rocephin and non-lethal to mice. |
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| Keywords: | bovine Helcococcus ovis isolation and identification 16S rRNA |
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