| 甜叶菊组织培养条件的研究Ⅰ．外植体、激素浓度、琼脂浓度对愈伤组织形成及芽诱导的影响 |
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| 引用本文： | 沈秀丽,徐仲.甜叶菊组织培养条件的研究Ⅰ．外植体、激素浓度、琼脂浓度对愈伤组织形成及芽诱导的影响[J].中国糖料,1996(3). |
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| 作者姓名： | 沈秀丽 徐仲 |
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| 作者单位： | 东北农业大学 |
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| 摘 要： | 以茎尖为外植体进行甜叶菊的组织培养，比以叶柄、叶片、茎段、下胚轴为外植体，愈仿组织诱导率、出芽率都高；在ＭＳ基本培养基上，适合茎尖生长、分化的激素浓度为０．５ｍｇ／ＬＢＡ、０．５ｍｇ／ＬＮＡＡ；琼脂浓度为０．７％
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| 关 键 词： | 甜叶菊，组织培养，外植体，激素 |
Studies on Conditions of Stevia rebaudiana bertoni Tissue CultureI.Effect of External plant and Concentrations of Hormoneand Agarose on Callus Formation and Germination |
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| Shen Xiuli et al.Studies on Conditions of Stevia rebaudiana bertoni Tissue CultureI.Effect of External plant and Concentrations of Hormoneand Agarose on Callus Formation and Germination[J].Sugar Crops of China,1996(3). |
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| Authors: | Shen Xiuli |
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| Abstract: | Stem apex used as external plant.the higher induced rate of callus and the higher germi-nation rate could be obtained than petiole,leaf,Stem and hypocotyl used as external plant inStevia rebaudiana bertoni tissue culture. On MS minimal medium,suitable hormone concen-tration for stem apex growth and its differentiation were 0.5mg/L BA plus 0.5mg/L NAA,and the agar concentration wab 0.7%. |
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| Keywords: | Stevia rebaudiana bertoni Tissuce culture External plant Hormone |
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