水分胁迫下小麦S 腺苷甲硫氨酸合成酶基因的半定量表达模式分析

为了解小麦S-腺苷甲硫氨酸合成酶(S-adenosylmethionine synthetase,SAMS) 基因(SAMS)在抗旱节水中的功能,以小麦品种陕229为材料,以本实验室克隆的普通小麦SAMS基因的序列设计引物,利用半定量RT-PCR方法,对小麦叶片中SAMS基因在正常供水及PEG 6000模拟水分胁迫12、24、48、60、78 h以及复水3、6、12 h时的表达模式进行分析.结果表明,小麦SAMS基因在正常生长情况下有一定量的表达,在水分胁迫早期(PEG 6000胁迫12、24、48 h)诱导上调表达,水分胁迫程度严重时(PEG 6000胁迫60、78 h)表达受到抑制,表达量低于对照,复水后(3、6 h)上调表达,复水18 h后表达量下调至对照水平.说明小麦SAMS基因的表达受水分胁迫及水分胁迫后复水诱导,是小麦抗旱节水的摘要: 为了解小麦S-腺苷甲硫氨酸合成酶(S-adenosylmethionine synthetase,SAMS) 基因(SAMS)在抗旱节水中的功能,以小麦品种陕229为材料,以本实验室克隆的普通小麦SAMS基因的序列设计引物,利用半定量RT-PCR方法,对小麦叶片中SAMS基因在正常供水及PEG 6000模拟水分胁迫12、24、48、60、78 h以及复水3、6、12 h时的表达模式进行分析.结果表明,小麦SAMS基因在正常生长情况下有一定量的表达,在水分胁迫早期(PEG 6000胁迫12、24、48 h)诱导上调表达,水分胁迫程度严重时(PEG 6000胁迫60、78 h)表达受到抑制,表达量低于对照,复水后(3、6 h)上调表达,复水18 h后表达量下调至对照水平.说明小麦SAMS基因的表达受水分胁迫及水分胁迫后复水诱导,是小麦抗旱节水的关键基因.

Expression Analysis of Wheat S adenosylmethionine Synthetase Gene during Water Stress and Re watering by Semi quantitative RT PCR

To study the functions of wheat S-adenosylmethionine synthetase(SAMS) gene in drought-tolerance and water use efficiency, the semi-quantitative RT-PCR was used to investigate the expression patterns of SAMS gene in the leaves of wheat seedling of Shaan 229 during PEG-simulated water stress and re-watering after serious water stress. The results showed that the expressions of SAMS were up-regulated at 12, 24 and 48 h of 0.5 Mpa PEG 6000 simulated water stress, then were down-regulated at 60 and 78 h of 0.5 Mpa PEG 6000 simulated water stress to less than normal level, and were up-regulated again at 3 and 6 h of re-watering, decreased again after 18 h of re-watering to the normal level. The expression patterns of wheat SAMS during water stress and re-watering after serious water stress suggested that this gene might be involved in the regulations of wheat to water stress and recovery, and was one of the key genes for the improvement for water use efficiency in wheat.