大鼠模型研究乳酸菌调节血脂作用

目的:探讨乳酸菌制剂对高脂血症模型大鼠血脂水平的影响.方法:采用高脂饲料喂养SD大鼠建立预防性高脂血症模型,将实验大鼠分成正常对照组、模型对照组、6#动物双歧杆菌低剂量组、中剂量组和高剂量组、Qq08菌低剂量组、中剂量组和高剂量组.连续灌胃给药45d,观察两株乳酸菌制剂对高脂血症大鼠血清甘油三酯(TG)、总胆固醇(TC)和高密度脂蛋白胆固醇(HDL-C)的影响.结果:与正常对照组比较,高脂饲料连续喂养45d后,模型对照组大鼠血清TG和TC显著升高(P＜0.01);与模型对照组比较,给药45d后,1)6#动物双歧杆菌低剂量组TG和TC显著降低(P＜0.05),中高剂量组TG和TC极显著地降低(P＜0.01)；2)Qq08菌低剂量组TG和TC显著降低(P＜0.05),Qq08菌中剂量组和高剂量组血清TG和TC极显著地降低(P＜0.01)；3)各剂量组大鼠血清HDL-C无显著性差异.结论:两株乳酸菌制剂都具有辅助降血脂的作用.

Blood Lipid Regulation of Lactic Acid Bacteria in Rat Models of Hyperlipidemia

Objective: To investigate the effects of two lactic acid bacteria (LAB) preparations (pure cultures ofBifidobacterium animalis No.6 and Bifidobacterium animalis.subsp.lactis Bl Qq08) on plasma lipid levels of hyperlipidemicrats. Methods: SD rats were fed high fat diet to establish preventive hyperlipidemia models. SD rats were divided into thefollowing groups: normal control, model control, low, middel and high dose Bifidobacterium animalis No.6, and low, middeland high dose Bl Qq08. After oral administration for 45 consecutive days, the effects of both LAB preparations on serumtriglyceride (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-c) in hyperlipidemic rat models wereobserved. Results: Compared with normal control group, serum TG and TC in model control group increased significantly(P＜0.01) after 45-days high-fat feeding. Compared with model control group, serum TG and TC in low dose group ofBifidobacterium animalis No.6 fell significantly (P＜0.05), and the two indicators exhibited an extremely significantdecrease in middle dose and high dose group of Bifidobacterium animalis No.6. Consistent results were found whencomparing different dose groups of Bl Qq08 and model control group. Howeve, no significant difference in serum HDL-cexisted among three dose groups for each LAB prepration. Conclusion: Both LAB preprations help reduce blood lipids.