| 固相萃取-高效液相色谱法测定乳制品中三氯蔗糖 |
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| 引用本文： | 杜彦山,杨刚,夏静,曹梅荣,张岩. 固相萃取-高效液相色谱法测定乳制品中三氯蔗糖[J]. 乳业科学与技术, 2013, 36(2): 27-29. DOI: 10.15922/j.cnki.jdst.2013.02.007 |
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| 作者姓名： | 杜彦山 杨刚 夏静 曹梅荣 张岩 |
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| 作者单位： | 河北省食品质量监督检验研究院,河北石家庄,050091 |
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| 摘 要： | 建立乳制品中三氯蔗糖的固相萃取高效液相色谱检测方法.采用0.2％乙酸水溶液沉淀蛋白,提取三氯蔗糖,然后通过C18固相萃取柱进行净化浓缩后,采用高效液相色谱-蒸发光散射检测仪进行检测,该方法0.1～0.8g/L范围内以三氯蔗糖进样质量的对数和相应峰面积的对数做标准曲线,R2为0.9991,相关性良好,加标回收率在94％～102％之间,检出限为2.0mg/L(RSN=3).
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| 关 键 词： | 乳制品；三氯蔗糖；高效液相色谱；蒸发光散射检测器；固相萃取 |
Determination of Sucralose in Dairy Foods by SPE-HPLC |
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| DU Yan-shan , YANG Gang , XIA Jing , CAO Mei-rong , ZHANG Yan. Determination of Sucralose in Dairy Foods by SPE-HPLC[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2013, 36(2): 27-29. DOI: 10.15922/j.cnki.jdst.2013.02.007 |
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| Authors: | DU Yan-shan YANG Gang XIA Jing CAO Mei-rong ZHANG Yan |
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| Affiliation: | (Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050091, China) |
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| Abstract: | A high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD)was developed for the determination of sucralose in dairy foods. Sucralose was extracted by precipitating samples with 0.2%acetic acid solution, cleaned up on a C18 solid phase extraction cartridge and concentrated prior to HPLC-ELSD analysis. Therelationship between logarithmic peak area and logarithmic sucralose concentration was linear over the range of 0.1 to 0.8 g/L, with acorrelation coefficient (R2) of 0.9991. The recoveries of sucralose from spiked milk powder and liquid milk ranged between94% and 102%. The limit of detection was 2 mg/L (RSN = 3). |
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| Keywords: | dairy products；sucralose；HPLC；ESLD；SPE |
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