| 液相色谱-串联质谱法测定贝类毒素软骨藻酸的残留 |
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| 引用本文： | 宋琍琍,张海琪,候镜德,何欣.液相色谱-串联质谱法测定贝类毒素软骨藻酸的残留[J].水产学报,2008,32(6):950-956. |
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| 作者姓名： | 宋琍琍 张海琪 候镜德 何欣 |
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| 作者单位： | 1. 浙江省水产质量检测中心,浙江,杭州,310012
2. 浙江大学分析测试中心,浙江,杭州,310027 |
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| 基金项目： | 浙江省重点科研社会发展项目（2007C23081） |
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| 摘 要： | 建立了液相色谱-串联质谱测定贝类毒素软骨藻酸残留的检测方法。样品经50%甲醇提取，LC-SAX柱净化，然后选用电喷雾离子源（ESI），在正离子、多反应监测方式（MRM）模式下进行定性，以外标法进行定量。方法的定量限为0.10μg/g，工作曲线的线性范围为0.10μg/g～8.00μg/g。在添加浓度为0.10μg/g～4.00μg/g的范围内，软骨藻酸的平均回收率范围为79.8%~92.2%，RSD范围为4.90%~9.46%。
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| 关 键 词： | 贝类 软骨藻酸 液相色谱串联质谱 多反应监测 |
| 收稿时间： | 5/9/2008 12:00:00 AM |
| 修稿时间： | 2008/7/29 0:00:00 |
High-performance liquid chromatography-tandem mass spectrometry for the determination of residue of domoic acid in shellfish |
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| SONG Lili,ZHANG Haiqi,HOU Jinde and HE Xing.High-performance liquid chromatography-tandem mass spectrometry for the determination of residue of domoic acid in shellfish[J].Journal of Fisheries of China,2008,32(6):950-956. |
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| Authors: | SONG Lili ZHANG Haiqi HOU Jinde and HE Xing |
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| Institution: | SONG Li-li1,ZHANG Hai-qi1,HOU Jing-de2,HE Xin1(1.Zhejiang Fishery Products Quality Testing Center,Hangzhou 310012,China,2.Center of Analysis , Measurement,Zhejiang University,Hangzhou 310027,China) |
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| Abstract: | Contamination of shellfish products by different biotoxins is one of the most important issues leading to the concern of food safety,which directly related to the health of seafood consumers.Even miniscule doses of some algal toxins,such as domoic acid(DA)or saxitoxin,can cause severe illness or death in humans.Therefore,it's important to carry on the reseaches on biotoxins contamination in shellfish,especially in China,where the shellfish mariculture industry has become more and more important.Detection me... |
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| Keywords: | shellfish domoic acid high-performance liquid chromatography-tandem mass spectrometry multiple reaction monitoring(MRM) |
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