添加消泡剂对光合细菌生长和产氢量的影响

消泡剂可以减少光合细菌产氢过程中泡沫的生成，防止泡沫对管道及外围设备的腐蚀。该文以磷酸三丁酯、十八醇、菜籽油和十二烷基苯磺酸钠作消泡剂研究了它们对光合细菌生长和产氢的影响，结果表明菜籽油和十八醇具有较好的消泡能力，并对光合细菌的生长和产氢有促进作用，其产氢体积分数分别比对照提高了5.4%和3.95%。磷酸三丁酯对光合细菌的生长有明显的抑制作用，十二烷基苯磺酸钠则降低光合细菌的增殖速度，使菌体达到最大菌体浓度的时间延迟了48 h，并使产氢体积分数下降了17.88%。菜籽油适宜作物光合产氢过程的消泡剂，建议其经济添加体积分数为0.05%。

Effects of defoamers on growth and hydrogen production process of photosynthetic bacteria

In order to eliminate the hazards of foam in the process of hydrogen production by photosynthetic bacteria (PSB), tributyl phosphate, stearyl alcohol, rapeseed oil and sodium dodecylbenzene sulfonate were used as defoamers in the experiments. The results showed the hydrogen yield increased with addition of rapeseed oil and stearyl alcohol by 5.4% and 3.95%, and rapeseed oil and stearyl alcohol had better capability to decrease the foam than the others. The growth of PSB was inhibited by tributyl phosphate which was covered the surface of culture medium. To reach max cell concentration, the growth time of PSB increased by 48 h and hydrogen yield decreased by 17.88% when sodium dodecylbenzene sulfonate was used as the defoamer. The rapeseed oil economy adding amount was 0.05% when it was covered the surface of culture medium in the process of hydrogen production by PSB.