| 利用DAS-ELISA检测侵染观赏百合黄瓜花叶病毒的研究 |
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| 引用本文： | 梁巧兰,魏列新,徐秉良. 利用DAS-ELISA检测侵染观赏百合黄瓜花叶病毒的研究[J]. 甘肃农业大学学报, 2006, 41(1): 61-64 |
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| 作者姓名： | 梁巧兰 魏列新 徐秉良 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 国家科技部资助(2001EA86005)，兰州市科委(03-1-39)共同资助 |
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| 摘 要： | 从制备的黄瓜花叶病毒———观赏百合分离物(CMV-Li)抗血清中提纯了IgG,并用过碘酸钠法进行辣根过氧化物酶(HRP)标记,建立了检测CMV-Li的DAS-ELISA(double antibody sandwich-enzyme-linked immumnosor-bent assay)系统,利用此检测系统并结合生物学方法,对临洮切花百合田间病样和百合不同部位带毒量进行了检测,结果发现CMV-Li的侵染率为23.81%,叶片的带毒量明显高于花和鳞茎.
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| 关 键 词： | 黄瓜花叶病毒 观赏百合 DAS-ELISA 检测 |
| 文章编号： | 1003-4313（2006）01-0061-04 |
| 收稿时间： | 2005-07-22 |
| 修稿时间： | 2005-07-22 |
Detection of cucumber mosaic virus on ornamental lily by double antibody sandwich ELISA |
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| LIANG Qiao-lan,WEI Lie-xin,XU Bing-liang. Detection of cucumber mosaic virus on ornamental lily by double antibody sandwich ELISA[J]. Journal of Gansu Agricultural University, 2006, 41(1): 61-64 |
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| Authors: | LIANG Qiao-lan WEI Lie-xin XU Bing-liang |
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| Affiliation: | College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China |
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| Abstract: | Immunoglobulin(IgG)from antiserum of ornamental lily that infected by cucumber raosaic virus was purified and marked with horseradish peroxidase(HRP) by sodium periodate.The method of DAS-ELISA for CMV-Li detection was developed.Some field samples of ornamental lily from Lintao and Yuzhong were detected by DAS-ELISA,the results showed that CMV-Li infected lily was a common pathogen of lily.About 23.85 % of detected lily samples both in Lintao and Yuzhong were infected by CMV.Meanwhile,the concentration of CMV-Li in ornamental lily leave was higher than that in flowers and bulbs. |
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| Keywords: | cucumber mosaic virus ornamental lily DAS-ELISA detection |
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