13/17罗伯逊易位猪CMYA 3基因多态性分析

目的]对13/17罗伯逊易位杂合子猪2n=36,rob（13;17）]互交后代中的148个个体进行了CMYA3基因的多态性分析。方法]采用PCR-RFLP方法。结果]通过PCR扩增所有个体均能获得507bp的目的片段,PCR产物经限制性内切酶Bsh1236I酶切,琼脂糖凝胶电泳结果表明该基因在此群体中具有A和B2个等位基因,基因频率分别为0.699和0.301;具有AA、AB和BB3种基因型,基因型频率分别为0.615、0.169和0.216。因此,该试验猪群中,A为优势等位基因,AA基因型频率最大。结论]该研究为13/17罗伯逊易位猪的分子育种和标记辅助选择提供理论依据。

Polymorphisms of CMYA3 Gene in 13/17 Robertsonian Translocation Pigs

Objective] The aim was to study the polymorphism of CMYA 3 gene in 148 pigs of hybrid offspring of 13/17 robertsonian translocation heterozygous pigs 2n=37, rob (13; 17)] intercrossing. Method] PCR-RFLP method was adopted. Result] A 507 bp fragment of CMYA3 gene was obtained by PCR amplification, and then amplification product was digested by restriction nuclease Bsh1236Ⅰ and detected by agarose gel electrophoresis. As a result, both alleles (A and B)of the loci were found in the population. The frequencies of allele A and B were 0.699 and 0.301. The genotype frequencies of AA, AB and BB were 0.615,0.169 and 0.216 respectively. The frequencies of allele A and genotype AA were significantly higher than allele B and genotype BB in populations. Result] The study will provide theoretical basis for molecular breeding and marker-assisted selection of 13/17 robertsonian translocation pigs.