| 小白菜和菜薹细胞质雄性不育类型的分子鉴定 |
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| 引用本文： | 周国林,林处发,胡侦华,等. 小白菜和菜薹细胞质雄性不育类型的分子鉴定[J]. 长江蔬菜, 2011, 0(4): 21-24. DOI: 10.3865/j.issn.1001-3547.2011.04.006 |
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| 作者姓名： | 周国林 林处发 胡侦华 等 |
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| 作者单位： | 周国林,林处发,胡侦华,邓耀华,汪爱华,ZHOU Guolin,LIN Chufa,HU Zhenhua,DENG Yaohua,WANG Aihua(武汉市蔬菜科学研究所,430065);丁毅,DING Yi(武汉大学植物发育生物学教育部重点实验室/武汉大学生命科学学院) |
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| 基金项目： | 现代农业产业技术体系建设专项资金 |
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| 摘 要： | 通过检索NCBI核苷酸数据库获得orf 138和orf 224开放阅读框序列,依据其保守序列设计2对引物,对几种来源不同的小白菜和菜薹细胞质不育系及其保持系进行PCR扩增.orf 138引物在小白菜和菜薹细胞质雄性不育系中均扩增出573 bp的片段,orf224引物只在一个菜薹雄性不育系中扩增出653 bp的片段.同源...
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| 关 键 词： | 小白菜 菜薹 细胞质雄性不育 |
Molecular Identification of Cytoplasmic Male Sterile Types Non-heading Chinese Cabbage and Chinese Flowering Cabbage |
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| ZHOU Guolin,LIN Chufa,HU Zhenhua,DING Yi,DENG Yaohua,WANG Aihua. Molecular Identification of Cytoplasmic Male Sterile Types Non-heading Chinese Cabbage and Chinese Flowering Cabbage[J]. Journal of Changjiang Vegetables, 2011, 0(4): 21-24. DOI: 10.3865/j.issn.1001-3547.2011.04.006 |
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| Authors: | ZHOU Guolin LIN Chufa HU Zhenhua DING Yi DENG Yaohua WANG Aihua |
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| Affiliation: | ZHOU Guolin1,LIN Chufa1,HU Zhenhua1,DING Yi2,DENG Yaohua1,WANG Aihua1(1.Wuhan Vegetable Science Research Institute,430065,2.College of Life Science/Key Laboratory of Plant Developmental Biology,Ministry of Education,Wuhan University) |
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| Abstract: | In order to identify the cytoplasmic male sterile(CMS) types in non-heading Chinese cabbage(Brassica campestris L.ssp.chinensis Makino) and Chinese flowering cabbage(B.campestris L.var.purpurea Baileysh),two pairs primers were designed based on the conserved domains of the sequences of orf 138 and orf 224 genes were got by searching the nucleotide databases of NCBI.The result indicated that a 573 bp specific fragment was produced in all the CMS lines by amplification with the orf 138 primer.The sequence ali... |
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| Keywords: | Non-heading Chinese cabbage Chinese flowering cabbage Cytoplasmic male sterile |
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