| 细叶桉ISSR-PCR体系的建立与优化 |
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| 引用本文： | 田华,张党权,谭晓风,黄青云,邓顺阳,樊绍刚,谷振军.细叶桉ISSR-PCR体系的建立与优化[J].经济林研究,2009,27(1). |
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| 作者姓名： | 田华 张党权 谭晓风 黄青云 邓顺阳 樊绍刚 谷振军 |
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| 作者单位： | 中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004 |
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| 基金项目： | 湖南省长沙市科技计划重点项目 |
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| 摘 要： | 细叶桉是我国近年来成功引种的耐寒桉树种类之一,但其种源关系难以区分,不利于后续新品种的培育.为给后续的分子水平上鉴定细叶桉种源关系打基础,以细叶桉叶片基因组DNA为材料.分析了能够影响细叶桉ISSR-PCR的主要参数如模板DNA、引物浓度、dNTPs、Mg2+浓度、TaqDNA聚合酶用量、退火温度等6个因素对扩增结果的影响情况,建立了适用于细叶桉ISSR-PCR的反应体系和扩增条件.
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| 关 键 词： | 细叶桉 反应体系 扩增条件 |
Establishment and Optimization of ISSR-PCR System of Eucalyptus Teretticornis |
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| TIAN Hua,ZHANG Dang-quan,TAN Xiao-feng,HUANG Qing-yun,DENG Shun-yang,FAN Shao-gang,GU Zhen-jun.Establishment and Optimization of ISSR-PCR System of Eucalyptus Teretticornis[J].Economic Forest Researches,2009,27(1). |
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| Authors: | TIAN Hua ZHANG Dang-quan TAN Xiao-feng HUANG Qing-yun DENG Shun-yang FAN Shao-gang GU Zhen-jun |
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| Institution: | The Key Lab of Non-wood Forest Products of Forestry Ministry;Central South University of Forestry and Technology;Changsha 410004;Hunan;China |
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| Abstract: | Eucalyptus teretticornis is one cold-resistant species of Eucalyptus which had been successfully introduced into China in recent years,but its provenance relations were difficult to distinguish,which was disadvantageous to the breeding of follow-up new variety.In order to lay the foundation for identification of Eucalyptus teretticornis provenance relations on molecular level,effect of six ISSR-PCR parameters of Eucalyptus teretticornis on amplification results were analyzed using genomic DNA of Eucalyptus ... |
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| Keywords: | ISSR-PCR |
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