| 苹果果实中蛋白质磷酸化对淀粉酶的激活 |
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| 引用本文： | 张凌云,张娜,潘秋红,段长青,张大鹏. 苹果果实中蛋白质磷酸化对淀粉酶的激活[J]. 园艺学报, 2003, 30(4): 455-459 |
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| 作者姓名： | 张凌云 张娜 潘秋红 段长青 张大鹏 |
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| 作者单位： | (中国农业大学果树分子发育实验室，植物生理生化国家重点开放实验室，北京100o94) |
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| 基金项目： | 国家自然科学基金项目(30070532,39730340);国家重点基础研究计划资助项目(G1999011700) |
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| 摘 要： | 用红富士苹果进行的研究表明，果实淀粉酶测定介质中加入ATP可显著激活淀粉酶活性，激活的幅度与ATP浓度有关。结合蛋白激酶抑制剂试验，表明苹果果实淀粉酶受到蛋白质磷酸化的激活。关键词：中图分类号：S 661．1
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| 关 键 词： | 苹果 果实 淀粉酶 蛋白磷酸化 |
| 文章编号： | 0513-353X(2003)04-0455-02 |
| 修稿时间： | 2002-07-16 |
Amylase Activities are Stimulated by Protein Phosphorylation in Apple Fruit |
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| Zhang Lingyun,Zhang Na,Pan Qiuhong,Duan Changqing,and Zhang Dapeng. Amylase Activities are Stimulated by Protein Phosphorylation in Apple Fruit[J]. Acta Horticulturae Sinica, 2003, 30(4): 455-459 |
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| Authors: | Zhang Lingyun Zhang Na Pan Qiuhong Duan Changqing and Zhang Dapeng |
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| Affiliation: | Department of Physiology, Fourth Military Medical University, Xi'an 710032, China |
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| Abstract: | The experiment conducted with Red Fuji apple(Ma／us dom／st／ca Borkh)showed that in vitro activities of both a—amylases and amylases of apple fruit significantly increased when adding ATP into the reaction of amylase assay，and this amylase activation was shown to be ATP dose—dependent．W7，an inhibitor of calcium-dependent protein kinases．significantly inhibited this am ylase activation also in a dose—depe ndent．These data indicate that a—amylases and t3-amylases ale activated by protein phosphorylation in developing apple fruit． |
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| Keywords: | Apple Fruit Amylase Protein phosphorylation |
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