| 斑马鱼促性腺激素释放激素及相关肽基因的克隆与序列分析 |
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| 引用本文： | 谢保胜,段瑞君.斑马鱼促性腺激素释放激素及相关肽基因的克隆与序列分析[J].上海水产大学学报,2009,18(3). |
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| 作者姓名： | 谢保胜 段瑞君 |
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| 作者单位： | 青海大学生物科学系,青海,西宁,810016 |
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| 基金项目： | 青海省高校日元贷款人才培养项目 |
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| 摘 要： | 从斑马鱼脑组织提取总RNA,应用RT-PCR方法克隆促性腺激素释放激素(GnRH) cDNA,其长度为646 bp,包括一个258 bp开放阅读框;编码的GnRH前体为86个氨基酸残基,由一个信号肽、GnRH十肽和一个由蛋白水解位点(Gly-Lys-Arg)连接的促性腺激素释放激素相关肽(GAP)组成;其中信号肽和相关肽的长度分别为24和49个氨基酸.该cDNA编码的GnRH的前体氨基酸序列与其他物种的GnRH前体基本一致.表明物种间GnRH cDNA的蛋白编码区高度保守,而非编码区的保守性程度很低.进化分析发现,斑马鱼与鲤、鲫、拟鲤、黑头软口鲦等淡水鲤科鱼类的同源性较高.
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| 关 键 词： | 斑马鱼 促性腺激素释放激素 cDNA克隆 序列分析 |
Cloning and sequence analysis of gonadotropin-releasing hormone and GnRH associated peptide in Danio rerio |
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| XIE Bao-sheng,DUAN Rui-jun.Cloning and sequence analysis of gonadotropin-releasing hormone and GnRH associated peptide in Danio rerio[J].Journal of Shanghai Fisheries University,2009,18(3). |
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| Authors: | XIE Bao-sheng DUAN Rui-jun |
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| Abstract: | The total RNA was extracted from brain of zebra fish.The GnRH cDNA was amplified by RT-PCR method using isolated total RNA as template.The GnRH cDNA is 646 bp in length that contain an open reading fram(ORF) of 258 bp,which encode the 86 amino acids residues that including a signal peptide(SP),a GnRH decapeptide and a GnRH-associated peptide(GAP) which is linked by the processing site Gly-Lys-Arg sequence.The cGnRH cDNA encode SP of 24 amino acids residues and GAP of 49 amino acid residues.The sequence stru... |
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| Keywords: | Danio rerio gonadotropin-releasing hormone(GnRH) cDNA cloning sequence analysis |
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