| 青砖茶不同超滤组分抑制α-淀粉酶和脂肪酶活性研究 |
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| 引用本文： | 刘淑媛,赵书青,倪德江,陈玉琼.青砖茶不同超滤组分抑制α-淀粉酶和脂肪酶活性研究[J].华中农业大学学报,2017,36(6):99-104. |
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| 作者姓名： | 刘淑媛 赵书青 倪德江 陈玉琼 |
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| 作者单位： | 园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院,武汉,430070 |
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| 基金项目： | 国家自然科学基金项目（31270732）; 湖北省科技项目（2012DBA17001） |
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| 摘 要： | 结合膜超滤技术从青砖茶中分离提取α-淀粉酶和脂肪酶抑制活性组分,分析青砖茶汤直接超滤组分和青砖茶汤乙酸乙酯萃取物超滤分离组分对α-淀粉酶和脂肪酶活性的影响。结果表明,青砖茶5ku膜截留组分具有较强的α-淀粉酶抑制活性;100ku膜截留组分具有较强脂肪酶抑制活性。乙酸乙酯萃取物5ku膜截留组分具有更强的α-淀粉酶抑制活性,而其透过组分具有更强的脂肪酶抑制活性。膜超滤技术结合乙酸乙酯萃取法能更好地分离纯化青砖茶汤α-淀粉酶和脂肪酶抑制活性成分。
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| 关 键 词： | 青砖茶 超滤 α-淀粉酶 脂肪酶 抑制剂 |
| 收稿时间： | 2017/3/27 0:00:00 |
Ultra-filtrated liquors from water extract of Qingzhuan tea inhibiting activities of pancreatic or-amylase and lipase in vitro |
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| LIU Shuyuan,ZHAO Shuqing,NI Dejiang,CHEN Yuqiong.Ultra-filtrated liquors from water extract of Qingzhuan tea inhibiting activities of pancreatic or-amylase and lipase in vitro[J].Journal of Huazhong Agricultural University,2017,36(6):99-104. |
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| Authors: | LIU Shuyuan ZHAO Shuqing NI Dejiang CHEN Yuqiong |
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| Abstract: | Fractions with high inhibitory activities on α-amylase and lipase were isolated from water extracts of Qingzhuan tea via ultra-filtration technology.Results showed that the trapped fraction of 5 ku ultra-filtration isolated from water extracts had strong α-amylase inhibitory activity.The trapped fraction of 100 ku ultra-filtration isolated from water extracts had strong lipase inhibitory activity.The α-amylase and lipase inhibitory activity of trapped fraction of 5 ku ultra-filtration isolated from the ethyl acetate fraction was higher than that of the ethyl acetate fraction.It is indicated that ultra-filtration combining with ethyl acetate extraction can more effectively isolate fractions with high α-amylase and lipase inhibitory activities from water extracts of Qingzhuan tea. |
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| Keywords: | Qingzhuan tea ultra-filtration α-amylase lipases inhibitory |
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