HPLC法测定丹参保健茶中3种丹参酮含量

目的]用HPLC测定丹参保健茶中隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA的含量。方法]色谱条件为：色谱柱为Insertsil ODS-SP（4.6mm×250 mm,5μm）;流动相为乙腈-水（V∶V=62∶38）,等度洗脱;检测波长为270 nm;流速为1.0 ml/min;进样量为15μl,柱温30℃。结果]3种丹参酮成分得到较好地分离,丹参保健茶中隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA的平均回收率分别为98.2%、98.7%、98.4%（RSD值分别为1.20%、1.96%和1.74%,n=3）;样品中3种丹参酮含量分别为1.19、0.79和2.25 mg/g。结论]该方法稳定可靠、简便快速,可用于丹参保健茶的质量控制。

Determination of Three Tanshinones Content in Salviae miltiorrhizae Health Tea by HPLC

Objective]To establish a HPLC method for simultaneously determining three major tanshinones in Salviae miltiorrhizae health tea.Method]HPLC was employed to determine the content of each composition.The HPLC conditions were as follows: column of Insertsil ODS-SP(4.6 mm×250 mm,5 μm),mobile phase of a mixture of acetonitrile-water(V∶V=62∶38),detection wavelength of 270 nm,flow rate of 1.0 ml/min,sampling amount of 15 μl and column temperature of 30 ℃.Result]Three compositions were well-separated and the average recovery of cryptotanshinone,tanshinone Ⅰ,tanshinone Ⅱ A were 98.2%,98.7% and 98.4% respectively(RSD value of 1.20%,1.96% and 1.74%,n=3).The content of the tanshinones in health tea were 1.19,0.79 and 2.25 mg/g respectively.Conclusion]The method is simple and rapid,which can be used to control the quality of Salviae miltiorrhizae health tea.