|
|||||
|
|
Production of recombinant goldfish gonadotropins by baculovirus in silkworm larvae |
|
| Authors: | M Kobayashi T Morita K Ikeguchi G Yoshizaki T Suzuki S Watabe |
| Institution: | 1. Department of Biology, International Christian University, Tokyo, Japan 2. Department of Aquatic Biosciences, Tokyo, University of Fisheries, Tokyo, Japan 3. Department of Aquatic Bioscience, University of Tokyo, Tokyo, Japan 4. Katakura Industries Co., Ltd., Saitama, Japan |
| Abstract: | Recombinant gonadotropins (GTH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) of goldfish Carassius auratus were produced by baculovirus in silkworm larvae. Hemolymph containing recombinant FSH (rFSH) or LH (rLH) was collected from silkworm larvae, and its biological activity was examined in vivo using male goldfish and female bitterling Rhodeus ocellatus ocellatus. Injection of hemolymph containing rFSH or rLH induced milt production in male goldfish, whereas only rLH induced ovulation in female bitterling. These results suggest, that biologically active goldfish recombinant GTH could be applied for the induction of gonadal development in aquaculture fishes as a substitute of pituitary extract, if a method of large scale production is established. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |