Immunolocalization of steroidogenic enzymes (P450scc, P450c17, P450arom) in gonad of Japanese eel |
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Authors: | Shigeho Ijiri Norio Takei Shinji Andachi Kohei Yamauchi |
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Affiliation: | 1. Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan
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Abstract: | Antibodies against P450scc, P450c17 and P450arom were generated using recombinant proteins. In eel testis, P450scc and P450c17 were immunolocalized as clusters in Leydig cells. In vitellogenic eel ovary, P450scc and P450c17 immunoreactive cells were localized as clusters in the outer layer of the ovarian follicle. In contrast, P450arom seemed to be immunolocalized in the innermost follicle layer. |
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