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Partial cloning of 17B-HSD1 from the Nile tilapia ovary and its expression pattern during spawning cycle
Authors:LY Zhou  B Senthilkumaran  DS Wang  CC Sudhakumari  T Kobayashi  H Kajiura-Kobayashi  M Matsuda  M Yoshikuni  Y Nagahama
Institution:1. Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, 444-8585, Japan
2. Faculty of Life Sciences, Southwest China Normal University, 400715, Chongqing, China
3. CREST, Kawaguchi, Saitama, 332-0012, Japan
Abstract:A 548 bp partial cDNA fragment of 17β-hydroxysteroid dehydrogenase (17β-HSD1) was obtained by RT-PCR from the ovary of Nile Tilapia. The expression of 17β-HSD1 was high from 0 to 11 days after spawning, but there was a sharp decline at the day of spawning (day 14) indicating its involvement in ovarian cycle.
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