| 大花蕙兰高频再生培养体系的建立 |
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| 引用本文： | 苏琛.大花蕙兰高频再生培养体系的建立[J].四川林业科技,2011,32(2):49-51,15. |
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| 作者姓名： | 苏琛 |
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| 作者单位： | 襄樊职业技术学院,湖北襄樊,441021 |
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| 基金项目： | 大花蕙兰高频再生培养技术体系的建立技术研究 襄科技【2006】6号 |
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| 摘 要： | 以大花蕙兰茎尖为外殖体,以MS为基本培养基,探讨了外源激素对原球茎分化、增殖与不定根形成的影响。试验结果表明：采用MS＋6-BA 0.5 mg·L-1～1.0 mg.L-1＋NAA0.1 mg·L-1～0.3 mg·L-1＋AC（活性炭）2.0 g.L-1的培养基,对原球茎的分化及增殖效果好;采用1/2MS＋6-BA0.1 mg.L-1＋NAA0.5 mg·L-1＋AC2.0 g·L-1的培养基,50 d内生根率可达93.3%。在水苔或水苔：椰糠=1：1的基质中驯苗,成活率可达92%以上。
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| 关 键 词： | 大花蕙兰 茎尖 植株再生 |
A Study of the Procedure of Cymbidium hybridum Cultured by Higher Frequency Regeneration |
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| SU Chen.A Study of the Procedure of Cymbidium hybridum Cultured by Higher Frequency Regeneration[J].Journal of Sichuan Forestry Science and Technology,2011,32(2):49-51,15. |
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| Authors: | SU Chen |
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| Institution: | SU Chen(Xiangfan Vocational and Technology College,Xiangfan 441021,Hubei) |
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| Abstract: | Cymbidium hybridum tip stemps were used as the explants.Researches were conducted on the effects of external hormone on green globlar body differentiation multiplication and uncertain roots forming.The results have shown that in the medium of MS＋6-BA0.5 mg·L-1～1.0 mg·L-1＋NAA0.1 mg·L-1-0.3 mg·L-1＋AC2.0 g·L-1,the effect is good on green globlar body differentiation multiplication;in1/2MS＋6-BA0.1 mg·L-1＋NAA0.5 mg·L-1＋AC2.0 mg·L-1,the rooting rate can reach 93.3% during 50 days;if the sprouts are transplanted into the substrate in which the rate between pseudopodium or pseudopodium and coconut moss is 1：1,the survival can reach over 92%. |
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| Keywords: | Cymbidium hybridum Stemp tip Plantlet regeneration |
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