Ri质粒介导双价抗真菌病基因转化番茄的初步研究

以番茄新品种T431、1911、0701为材料，以9天苗龄的叶片为受体，通过农杆菌Ri质粒介导，将几丁质酶基因和?-1，3葡聚糖酶基因转化番茄。共获得再生植株100株，经PCR技术检测，导入几丁质酶基因和?-1，3葡聚糖酶基因的植株分别为27株和13株，转化率为27% 和13%；两个目的基因同时导入的植株10株，转化率为10%。PCR-Southern检测呈阳性，表明外源基因已经整合到番茄基因组中。对9株双转化的植株进行RT-PCR检测，几丁质酶基因和?-1，3葡聚糖酶基因的阳性植株分别为5株和4株，证明转化的基因已表达。

Study on Genetic Transformation of Tomato Expressing Bivalent Anti-fungal Disease Gene Mediated by Ri Plasmid

New tomato varieties T431, 1911 and 0701 were used as the materials, chitinase gene and β -1, 3-glucanase gene were transformed into tomatoes by Agrobacterium-mediated transformation. A total of 100 regeneration plants were obtained. In these regenerated plants, 27 and 13 plants appeared respectively to be positive in PCR test with chitinase gene and β -1,3-glucanase gene primer. The transferring frequency of foreign gene was 27% and 13% separately. If the two genes were done simultaneously, 10 plants appeared to be positive in PCR test with a transferring frequency of 10%. PCR-Southern analysis of these plants showed that they produced positive hybridization, indicating that the chitinase gene and β -1,3-glucanase gene have been conveyed and integrated into genome of these plants.