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陕西小麦 Glu A3 Glu B3位点等位变异的检测和分析
引用本文:梁 强,王晓龙,张晓科,张 晶,张同兴,冯 毅,王 瑞,王宏礼. 陕西小麦 Glu A3 Glu B3位点等位变异的检测和分析[J]. 麦类作物学报, 2011, 31(5): 859-864
作者姓名:梁 强  王晓龙  张晓科  张 晶  张同兴  冯 毅  王 瑞  王宏礼
作者单位:(1.西北农林科技大学农学院/国家小麦改良中心杨凌分中心,陕西杨凌712100; 2.陕西省岐山县农技中心,陕西岐山722400)
基金项目:“948”项目(2006G2);小麦产业技术体系建设专项(nycytx03);西北农林科技大学唐仲英育种基金项目。
摘    要:低分子量谷蛋白亚基(LMWGS)与小麦品质密切相关。为了给陕西小麦的品质改良提供参考依据,采用STS分子标记,检测了175份陕西小麦品种(系) GluA3 GluB3位点的等位变异组成。结果表明,陕西小麦 GluA3位点存在4种等位变异,即 GluA3a GluA3b GluA3c GluA3d,分别占12.6%、1.7%、58.3%和27.4%; GluB3位点存在8种等位变异,即 GluB3a GluB3b GluB3d GluB3e GluB3f GluB3g GluB3i GluB3j,分别占4.6%、2.9%、45.7%、0.6%、2.9%、8.5%、4.0%和30.8%。在陕西不同地区小麦之间,两个位点等位变异的种类、组合及其分布比例存在差异,这可能与地区间不同的自然地理环境、饮食习惯、育种目标及亲本选择有关。

关 键 词:小麦;LMWGS;STS标记; GluA3 GluB3

Idetection and Analysis of Allelic Variations at Glu A3and Glu B3Loci in Shaanxi Wheats
LIANG Qiang,WANG Xiao long,ZHANG Xiao ke,ZHANG Jing,ZHANG Tong xing,FENG Yi,WANG Rui,WANG Hong li. Idetection and Analysis of Allelic Variations at Glu A3and Glu B3Loci in Shaanxi Wheats[J]. Journal of Triticeae Crops, 2011, 31(5): 859-864
Authors:LIANG Qiang  WANG Xiao long  ZHANG Xiao ke  ZHANG Jing  ZHANG Tong xing  FENG Yi  WANG Rui  WANG Hong li
Abstract:Quality properties of wheat (Triticum aestivum L.) are strongly affected by low molecular weight glutenin subunits (LMW GS). It is very important for improvement of wheat qualities to study the LMW GS compositions and distribution in Shaanxi wheat cultivars and advanced lines. STS markers were used to detect the allelic variations of LMW GS at Glu A3and Glu B3in 175 wheat cultivars and advanced lines from Shaanxi Province. Four allelic variations of LMW GS were detected at Glu A3locus in Shaanxi wheats, i.e. Glu A3a, Glu A3b, Glu A3cand Glu A3d, accounting for 12.6%, 1.7%, 58.3% and 27.4%, respectively. And eight allelic variations were found at Glu B3locus, i.e. Glu B3a, Glu B3b, Glu B3d, Glu B3e, Glu B3f, Glu B3g, Glu B3iand Glu B3j, accounting for 4.6%, 2.9%, 45.7%, 0.6%, 2.9%, 8.5%, 4.0% and 30.8%, respectively. There were different types and combinations of LMW GS allelic variations in three diverse regions of Shaanxi Province, which was likely related to the regional eating habits, wheat breeding programs and parental materials selected in wheat breeding.
Keywords:Triticum aestivum L.   LMW GS   STS markers   Glu A3   Glu B3
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