| 花生油酸/亚油酸(O/L)比值的AFLP分子标记筛选 |
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| 引用本文： | 丁锦平.花生油酸/亚油酸(O/L)比值的AFLP分子标记筛选[J].河南农业科学,2011,40(6):63-65. |
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| 作者姓名： | 丁锦平 |
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| 作者单位： | 商丘师范学院生命科学系，植物与微生物互作重点实验室，河南商丘476000 |
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| 摘 要： | 通过采用不同的酶切组合,即EcoRⅠ/MseⅠ组合240对引物、PstⅠ/MseⅠ组合96对引物、PstⅠ/TaqⅠ组合96对引物,分别对花生高油酸品种Sunoleic95R和低油酸品种汕油162进行AFLP分析.分析了两亲本之间的差异,回收了72条AFLP多态性条带,在所选定高和低O/L比值亲本材料之间建立了AFLP...
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| 关 键 词： | 花生 油酸/亚油酸(O/L) 扩增片段长度多态性 酶切组合 |
The AFLP Analysis of Oleic/Linoleic(O/L) Ratio in Peanut |
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| DING Jin-ping.The AFLP Analysis of Oleic/Linoleic(O/L) Ratio in Peanut[J].Journal of Henan Agricultural Sciences,2011,40(6):63-65. |
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| Authors: | DING Jin-ping |
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| Institution: | DING Jin-ping(Key Laboratory of Plant-Microbe Interactions,Department of Life Science,Shangqiu Normal University,Shangqiu 476000,China) |
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| Abstract: | Totally 240 EcoR Ⅰ/Mse Ⅰ AFLP selective primer pairs,96 Pst Ⅰ/Mse Ⅰ AFLP selective primer pairs and 96 Pst Ⅰ/Taq Ⅰ AFLP selective primer pairs were used in the analysis of genotypic difference between two parental lines,namely Shanyou162 with low oleic content and Sunoliec95R with hight oleic content.The genotypic differences were found out in two parents.Totally 72 polymorphic AFLP bands were recovered from the PAGE plate,subsequent cloning and verification work were done later.The AFLP different fragment sequence database was established for the parent materials. |
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| Keywords: | Peanut Oleic/Linoleic(O/L) AFLP Enzyme combination |
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