| 鸡传染性贫血病毒vp3基因的原核表达及其免疫学活性分析 |
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| 引用本文： | 陆桂丽,王笑梅,高玉龙,高宏雷,祁小乐,王晓燕. 鸡传染性贫血病毒vp3基因的原核表达及其免疫学活性分析[J]. 中国预防兽医学报, 2006, 28(6): 650-652 |
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| 作者姓名： | 陆桂丽 王笑梅 高玉龙 高宏雷 祁小乐 王晓燕 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001;新疆农业大学,动物医学院,新疆,乌鲁木齐,830052
2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 将鸡传染性贫血病毒(CIAV)Vp3基因克隆入表达载体pET-28a中,然后转化大肠杆菌DH5α,经IPTG诱导,vp3基因以融合蛋白的形式高效表达。SDS-PAGE分析显示,表达的重组蛋白分子量约为18Ku。Westernblot分析表明,该蛋白可与CIAV阳性血清特异性反应。
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| 关 键 词： | 鸡传染性贫血病毒 vp3基因 原核表达 |
| 文章编号： | 1008-0589(2006)06-0650-03 |
| 收稿时间： | 2005-10-09 |
| 修稿时间： | 2005-10-09 |
Expression of chicken infectious anemia virus vp3 gene in Escherichia coli and analysis of immunological activity |
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| LU Gui-li,WANG Xiao-mei,GAO Yu-long,GAO Hong-lei,QI Xiao-le,WANG Xiao-yan. Expression of chicken infectious anemia virus vp3 gene in Escherichia coli and analysis of immunological activity[J]. Chinese Journal of Preventive Veterinary Medicine, 2006, 28(6): 650-652 |
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| Authors: | LU Gui-li WANG Xiao-mei GAO Yu-long GAO Hong-lei QI Xiao-le WANG Xiao-yan |
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| Abstract: | Chicken infectious anemia virus vp3 gene was cloned into an expression vector pET-28a.The resulting recombinant plasmid was named pET-28a-vp3.A His-tagged recombinant protein was expressed with high efficiency in E.coli transformed by pET-28a-vp3 after induction with IPTG.The fusion protein has an apparent molecular weight of about 18 Ku as indicated by SDS-PAGE analysis,and showed specific reaction with positive serum of CIAV in Western blot. |
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| Keywords: | chicken infectious anemia virus vp3 gene prokaryotic expression |
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