| 一种从感病叶片中高效提取条锈菌基因组DNA的方法 |
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| 引用本文： | 代君丽,于思勤,韩帅,种斌,李素芳,毛红彦,李洪连. 一种从感病叶片中高效提取条锈菌基因组DNA的方法[J]. 西北农业学报, 2009, 18(2): 304-306 |
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| 作者姓名： | 代君丽 于思勤 韩帅 种斌 李素芳 毛红彦 李洪连 |
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| 作者单位： | 1. 河南农业大学,植物保护学院,河南郑州,450002
2. 河南省植保植检站,河南郑州,450002 |
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| 基金项目： | 国家科技支撑计划重大项目 |
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| 摘 要： | 以CTAB/SDS法直接从感病叶片中提取条锈菌基因组DNA,该法与β-巯基乙醇法相比,提取DNA纯度和产量均较高,所提取DNA的产量大约为β-巯基乙醇法提取DNA产量的5～10倍.并且用所提取的DNA为模板进行PCR扩增能够获得条锈菌特异性条带.
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| 关 键 词： | 感病叶片 条锈菌 β-巯基乙醇 |
| 收稿时间： | 2008-06-24 |
| 修稿时间： | 2008-12-16 |
An Efficient Protocol for Isolating Genomic DNA of Puccinia striiformis f. sp. tritici from Infected Leaves |
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| DAI Junli,YU Siqin,HAN Shuai,CHONG Bin,LI Sufang,MAO Hongyan and LI Honglian. An Efficient Protocol for Isolating Genomic DNA of Puccinia striiformis f. sp. tritici from Infected Leaves[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(2): 304-306 |
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| Authors: | DAI Junli YU Siqin HAN Shuai CHONG Bin LI Sufang MAO Hongyan LI Honglian |
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| Affiliation: | College of Plant Protection, He''nan Agricultural University, Zhengzhou 450002, China;He''nan Plant Protection and Quarantine Station, Zhengzhou 450002, China;College of Plant Protection, He''nan Agricultural University, Zhengzhou 450002, China;College of Plant Protection, He''nan Agricultural University, Zhengzhou 450002, China;He''nan Plant Protection and Quarantine Station, Zhengzhou 450002, China;He''nan Plant Protection and Quarantine Station, Zhengzhou 450002, China;College of Plant Protection, He''nan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | |
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| Keywords: | CTAB/SDS |
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