大麦黄矮病毒-GAV在燕麦植株体内运动规律的初步研究

 利用RT-PCR方法研究了大麦黄矮病毒-GAV在燕麦植株内的移动规律。先将介体麦二叉蚜(Schizaphis graminum)在BYDV-GAV新鲜病叶上饲毒,再将获毒蚜虫放置到二叶期的健康燕麦植株接种48h,随后分期提取接种植株的第1~6片叶和根组织的总RNA,利用特异引物扩增BYDV-GAV的外壳蛋白(CP)基因以检测病毒在燕麦植株内的复制和移动。结果表明,在接种5d后,接种叶片(第2片叶)呈现阳性,接种7d后,植株新生的第4片叶被侵染,接种9d后,部分的第3片叶呈现阳性,至接种16d,几乎所有的叶片均呈现阳性。仅在接种的第5、7和9d收集的根组织呈现阳性,而所有的第1片叶均为阴性,可能是由于这些组织内病毒含量太低所致。本研究初步揭示了BYDV-GAV长距离运动的规律并且发现该病毒在燕麦根部从接种到系统发病都没有进行大量增殖,为今后进一步研究病毒运动机制选取适当的植物材料提供了基本信息。

Studies on the movement of Barley yellow dwarf virus-GAV in oat plant by RT-PCR

The movement of BYDV-GAV in oat plants was studied by RT-PCR.Healthy oat plants were inoculated with BYDV-GAV by vector Schizaphis graminum on the second leaf.The coat protein gene was amplified with total RNA from the first to sixth leaf and root of inoculated oat plants at different growth stages to test the replication and movement of the virus in the plants.The results showed that the second leaf(inoculated leaf) was identified as positive after inoculation for 5 days.After 7 days,the fourth leaves were infected,it was the new leaf at that stage.After 9 days,some of the third leaves were identified as positive.After 16 days almost all of the leaves were infected.The roots were identified as positive only at 5th,7th and 9th day respectively,and also all of the first leaves were always identified as negative,which might be due to the virus content in the plant was too low to detect.The movement of the virus in oat plant will provide basic information for selecting optimal tissue to study the movement mechanism.