性别控制胚胎玻璃化一步法稀释冷冻保存技术的研究

为了创立在用户庭院就能应用的玻璃化保存性别控制胚胎的冷冻剂稀释处理方法，采用细管内3种不同液体层构成（A、B、C）法，对新鲜分割胚一步法稀释后的存活能力进行了研究；其次用特定细管最小容量冷却法与上述3种不同液体层构成中效果较好的B法对玻璃化冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚进行一步法稀释后的存活能力予以对比。结果，3种不同液体层构成间胚胎的存活能力无显著差异，B法较高；应用特定细管最小容量冷却法在冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚一步法稀释后的存活能力均显著高于B法。结果表明，特定细管最小容量冷却法是更有效的保存方法。

Gender-controlled embryo freezingly-preserved technique by one-step vitrification dilution

For household users to establish simple antifreeze dilution disposal ways of applying gendercontrolled embryo freezingly-preserved technique by one-step vitrification dilution method, A, B, and C structure approaches of liquid layers in canaliculi were applied to investigate their survival capacity after embryos freshly-split by one-step dilution;and minimum capacity refrigerant method in specific canaliculus and above structure approach of liquid layer B were also applied to compare their survival capacity of frozen and melted embryos freshly split,and frozen and melted IVF embryos splited by one-step dilution. The resuits showed that there was no significant difference in three structure approach of liquid layers among which B was relatively higher; and it was considered that one-step dilution was an effective preservation method in that survival capacity with frozen and melted embryos freshly split, and survival capacity of frozen and melted IVF embryos split by one-step dilution by application minimum capacity refrigerant method with specific canaliculus were obviously higher than B.